法医学杂志

法医学杂志 ›› 1998, Vol. 14 ›› Issue (4): 203-208+.

• 论文 • 上一篇    下一篇

用气相色谱法/电子捕获检测器测定尿液中的三唑仑

刘伟   

  1. 司法部司法鉴定科学技术研究所!上海,200063
  • 发布日期:1998-08-25 出版日期:1998-08-28

Determination of triazolam in human urine by GC/ECD

LIU WEI (INSTITUTE OF FORENSIC SCIENCES, MINISTRY OF JUSTICE,PR. CHINA, SHANGHAI 200063)   

  • Online:1998-08-25 Published:1998-08-28

被引次数

12

摘要： 建立了用气相色谱／电子捕获检测器测定尿液中三唑仑含量的方法。2ml尿样在破性条件下用2ml×2氯仿提取净化后，60℃水浴下用空气吹干，残留物用环己烷定容溶解后，进气相色谱仪分析，三唑仑的保留时间为10．74min。最低检测限为0．5ng／ml，回收率为95．98％，变异系数为7．85％（n＝5）。在2～50ng／ml浓度范围内有良好的线性关系：A＝－67．9＋570．IC，r＝0．9939。

关键词: 三唑仑, 气相色谱法吨子捕获检测器, 尿液

Abstract: A GC/ECD method was established to determine triazolam in human urine qualitatively and quantitatively. The triazolam was extracted from 2ml urine with 2 x 2ml chloroform.The retention time of triazolam was 10. 74 min. The limit ofthe determination was 0. 5ng/ml. The recovery of the procedure in 5 ng/ml was 95.98%, CV = 7. 85% (n = 5). Thelimit of quantitation is 2 - 50ng/ml.

Key words: triazolam, GC/ECD, urine