法医学杂志

法医学杂志 ›› 1999, Vol. 0 ›› Issue (3): 135-137+.

• 论文 • 上一篇    下一篇

死后大鼠骨骼肌运动终板和肌纤维酶、免疫组化研究

王慧君;张春明;熊希凯;黄光照;武忠弼;   

  1. 同济医科大学超微病理学研究室,同济医科大学超微病理学研究室,同济医科大学人体解剖学教研室,同济医科大学法医学系法医病理学教研室,同济医科大学超微病理学研究室 湖北武汉 430030,湖北武汉 430030,湖北武汉 430030,湖北武汉 430030,湖北武汉 430030
  • 发布日期:1999-06-25 出版日期:1999-06-28

Enzymehistochemical and immunohistological changes of skeletal muscle motor end-plates and muscle fibers and their relation to the time of death

WANG HUIJUN, ZHANG CHUNMING, XIONG XIKAI, ET AL (DEPARTMENT OF ULTRASTRUCTURAL PATHOLOGY, RESEARCH CENTER OF EXPERIMENTAL MEDICINE, TONGJI MEDICAL UNIVERSITY, WUHAN 430030, P.R. CHINA)   

  • Online:1999-06-25 Published:1999-06-28

被引次数

4

摘要： 为进一步了解骨骼肌组织的死后变化特点和规律及其与死后时间(PMI)的关系,本文选用成年实验大鼠。系列观察了死后0～96小时(0～96hpm)骨骼肌运动终板和肌纤维在不同温度条件下AChE(乙酰胆碱酯酶),SDH(琥珀酸脱氢酶),LDH(乳酸脱氢酶),Ca~(2+)-ATPase(钙激活三磷酸腺苷酶)的酶组织化学变化及运动终板突触素(synaptophysin,SYN)的免疫组织化学变化,并使用图像处理系统对上述酶反应结果进行了定量分析和比较。结果发现,上述改变与PMI有明显的相关关系,其中运动终板AChE因位于突触间隙的特殊位置以及自身的耐活性质,反应明确,稳定性高,降解缓慢显示更清晰和持久的降解曲线,可用作为推断死亡时间的标志酶。

关键词: 骨骼肌, 运动终板, AchE, 酶组化, PMI

Abstract: In order to investigate the relationship between the retrograde changes of the skeletal muscle and the time of death in various postmortem intervals (PMI), a systemic study of the enzymehistochemical activity of ACHE, SDH, LDH, Ca~(2+)-ATPase and the immunohistochemical reaction of SYN in motor end-plates and muscle fibers was conducted in rats under different temperatures and at various PMI. The results were analyzed and compared by an image processing system. It was found that these changes were related to the PMI, especially AChE changes. The AChE could be used as a sign-enzyme of skeletal muscle to date death.

Key words: skeletal muscle, motor end- plate, AchE, enzymhistochemistry, PMI