法医学杂志 ›› 2005, Vol. 0 ›› Issue (1): 21-23+2.

• 论文 • 上一篇    下一篇

心肌缺血内皮素RT-PCR初步研究及其意义

马孟云,徐小虎   

  1. 深圳市公安局刑科所,汕头大学医学院 广东深圳518001 ,广东汕头515031
  • 发布日期:2005-02-25 出版日期:2005-02-28

Primarily Study and Significance of ET-1 During Ischemia with RT-PCR

MA MENG-YUN1,XU XIAO-HU2 (SHENZHEN INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY, SHENZHEN 518001,CHINA)   

  • Online:2005-02-25 Published:2005-02-28

摘要: 目的为探讨心肌缺血后内皮素基因在心肌组织内表达的变化。方法用RT-PCR法研究了心肌缺血60min后心肌内皮素的表达情况。结果发现RT-PCR结果显示缺血组和正常组均检出ET-1mRNA,但是二者之间有显著性差异,说明缺血可以诱导ET-1mRNA增高。结论本实验为ET-1免疫组织化学法研究奠定了分子基础。

关键词: 心肌缺血, 内皮素, RT-PCR, 大鼠

Abstract: Objective To explore the relationship between expression of endothelin in heart and ischemia. Methods Using the RT-PCR method, we enquiry changes of ET-1 RNA after 60min ischemia in hearts of rats. Results Although the two group appear positive, they are different significantly, which indicate that ischemia may lead to the increase of ET-1 mRNA. Conclusion The experiment, therefore, pave a way for immunochemical study.

Key words: ischemia, ET-1, RT-PCR, rat