法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (1): 24-26.

• 论文 • 上一篇    下一篇

人脑挫伤后HSP70和iNOS表达研究

张巍,汪德文,孙晓东,曹明,胡更奕,赵海南,王斌   

  1. 中国医科大学法医学院,中国医科大学法医学院,沈阳市公安局治安特警支队,新民市公安局,中国医科大学法医学院,沈阳市胸科医院,沈阳市监管支队看守所 辽宁沈阳110001沈阳市公安局交警支队,辽宁沈阳110003 ,辽宁沈阳110001 ,辽宁沈阳110003 ,辽宁新民110300 ,辽宁沈阳110001 ,辽宁沈阳110044 ,辽宁沈阳110031
  • 发布日期:2005-02-25 出版日期:2005-02-28

A Study on the Expression of HSP70 and iNOS after Human Brain Contusion

ZHANG WEI, WANG DE-WEN, SUN XIAO-DONG, ET AL. ( SCHOOL OF FORENSIC MEDICINE,CHINA MEDICAL UNIVERSITY, SHENYANG 110001,CHINA)   

  • Online:2005-02-25 Published:2005-02-28

摘要： 目的探讨人脑挫伤后不同时间内HSP70和iNOS表达的变化关系。方法应用免疫组织化学染色观察35例人脑挫伤组织HSP70和iNOS表达的变化规律。结果HSP70阳性细胞在即刻死亡组表达强度最大,伤后急剧下降,至24h达最低,以后逐渐恢复。iNOS阳性细胞表达强度在伤后48h升高至最大,以后逐渐下降,在伤后11d仍有弱表达。结论HSP70和iNOS免疫组化染色可以作为推断人脑挫伤经过时间的有效指标。

关键词: 脑挫伤, HSP70, iNOS, 免疫组织化学

Abstract: Objective To study the relationship between expression of HSP70, iNOS and traumatic brain contusion (TBI) in different posttraumatic intervals. Methods 35 samples of brain contusion were examined using immunohistochemecal staining to evaluate the expression of HSP70 and iNOS. Results Maximal HSP70 expression was found at 0h after brain contusion. The intensity of HSP70 staining decreased remarkably to the minimum at 24 h after TBI, then increased gradually. Expression of iNOS positive cells increased significantly and reached the maximum level 48h after TBI, then the expression decreased gradually from the 2nd day to the 11th day. Conclusion The changes of HSP70 and iNOS immunohstochemical staining can be used as a referential data for estimating time interval after human brain contusion.

Key words: brain contusion, HSP70, iNOS, immunohistochemistry