法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (2): 100-103+.

• 论文 • 上一篇    下一篇

呼和浩特等地区嗜尸性苍蝇mtDNA中COⅠ基因序列检测及法医学应用

蔡继峰,董建国,刘敏,应斌武,陶涛,潘洪富,张林,闫红涛,张嘉陵,廖志钢   

  1. 四川大学华西基础与法医学院,上海第二医科大学基础医学院病理学教研室,四川大学华西基础与法医学院,四川大学华西基础与法医学院,四川大学华西基础与法医学院,四川大学华西基础与法医学院,四川大学华西基础与法医学院,四川大学华西基础与法医学院,成都市人民检察院,四川大学华西基础与法医学院 四川成都610041 ,上海200025 ,四川成都610041 ,四川成都610041 ,四川成都610041 ,四川成都610041 ,四川成都610041 ,四川成都610041
  • 发布日期:2005-04-25 出版日期:2005-04-28

Forensic Applications of the Sequencing of Mitochondrial DNA Cytochrome Oxidase SubunitⅠGene for Sarcosaphagous Flies (Diptera) in Huhhot and Dunhuang District

CAI JI-FENG1, DONG JIAN-GUO2, LIU MIN1, ET AL. (1.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041,CHINA; 2.DEPARTMENT OF PATHOLOGY, SCHOOL OF PRECLINICAL MEDICINE, SHANGHAI SECOND MEDICAL UNIVERSITY, SHANGHAI 200   

  • Online:2005-04-25 Published:2005-04-28

摘要： 目的通过对嗜尸性苍蝇线粒体DNA(mtDNA)上细胞色素氧化酶辅酶Ⅰ(COⅠ)中278bp基因序列的分析,对其成虫及其幼虫、蛹、卵的种属进行鉴定,解决依据形态学方法难以鉴定的难题。方法随机分别采集呼和浩特和甘肃敦煌地区兔和猪尸体上的嗜尸性苍蝇、幼虫、蛹及其腹中的卵,提取其mtDNA,经PCR扩增、产物检测与纯化、测序等,进行序列分析和构建系统发育树。结果双翅目嗜尸性苍蝇的种内基因差异均数小于1%,种间差异均数大于3%,成虫与幼虫、蛹、卵之间无显著性差异。结论mtDNA上COⅠ序列分析均可以有效地对嗜尸性苍蝇及其幼虫、蛹、卵进行种属鉴定,方法快速、简便和精确,可作为法医学嗜尸性苍蝇种属鉴别的可靠方法。

关键词: 嗜尸性苍蝇, 种属鉴定, 线粒体DNA, 细胞色素氧化酶辅酶Ⅰ

Abstract: Objective To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs. Methods Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunitⅠ (COⅠ) gene in mtDNA was analysed by DNA sequencing, A neighbour-jioning tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package. Results A 278 base pairs region of the gene for COⅠencoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species. Conclusion It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the COⅠencoding gene of mtDNA.

Key words: Sarcosaphagous flies, species identification, mtDNA, COⅠ