法医学杂志

法医学杂志 ›› 2005, Vol. 0 ›› Issue (4): 283-285.

• 论文 • 上一篇    下一篇

D6S2418在中国(汉族)、泰国和德国人群中的遗传多态性

刘志勇,陈国弟,任广田,余琳,韩军鸽,王晶,廖淼,梁伟波,张林   

  1. 四川大学华西基础与法医学院法医物证教研室,四川大学华西基础与法医学院法医物证教研室,深圳市公安局盐田分局,四川大学华西基础与法医学院法医物证教研室,四川大学华西基础与法医学院法医物证教研室,四川大学华西基础与法医学院法医物证教研室,四川大学华西基础与法医学院法医物证教研室,四川大学华西基础与法医学院法医物证教研室,四川大学华西基础与法医学院法医物证教研室 四川成都610041 ,四川成都610041 ,广东深圳518082 ,四川成都610041 ,四川成都610041 ,四
  • 发布日期:2005-08-25 出版日期:2005-08-28

Research on the Genetic Polymorphisms of D6S2418 Locus in Chinese Han Ethnic, Thai and Germany Populations

LIU ZHI-YONG1,CHEN GUO-DI1, REN GUANG-TIAN2,ET AL. (1. WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041, CHINA; 2. CENTER OF FORENSIC SCIENCES, PUBLIC SECURITY BUREAU OF YANTIAN DISTRICT, SHENZHEN 518082,CHINA)   

  • Online:2005-08-25 Published:2005-08-28

摘要： 目的获得D6S2418基因座的群体遗传学数据,分析其基因频率在不同群体间的分布情况是否存在差异,并分析其在法医学中的应用价值。方法采用PCR、非变性聚丙烯酰胺凝胶电泳(PAGE)及银染技术分析中国成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群中D6S2418基因座的遗传多态性,获得三个群体D6S2418基因座的群体遗传学数据。结果从300份分别采自成都地区汉族、泰国曼谷地区泰国人群以及德国Maint地区德国人群三个群体的无血缘关系个体的静脉血,共发现9个等位基因,观测到31种基因型。观测杂和度为64%～81%,个人识别机率为84.2%～93.5%,经统计学检验,基因型的频率分布符合Hardy-Weinberg平衡定律。等位基因频率的分布在三个群体间有显著差异。结论D6S2418基因座的个人识别能力高,在法医学个人识别和亲子鉴定应用中有较高价值。

关键词: 短串联重复序列, PCR, D6S2418, 遗传多态性

Abstract: Objective To obtain the genetic data on STR locus D6S2418,and analyzed its genetic polymorphism in 3 different races (Han,Thai and Germany). Methods The blood samples from 300 unrelated individuals were analyzed by PCR,PAG electrophoresis and silver staining. Results 9 alleles and 31 genotypes were observed.The observed heterozygosity and the discrimination power were 64%-81% and 84.2%-93.5%,respectively.The genotype distributions of D6S2418 locus in 3 populations were in accordance with Hardy-Weinberg equilibrium and the difference of allelic frequency distributions among 3 populations was obvious. Conclusion The methods founded are easy to perform and sensitive. The discrimination power and exclusion chances of this locus are desirable for forensic analysis and application.

Key words: short tandem repeat, PCR, D6S2418, genetic polymorphism