法医学杂志

法医学杂志 ›› 2006, Vol. 0 ›› Issue (1): 18-20+9.

• 论文 • 上一篇    下一篇

过敏性休克死亡豚鼠器官中IgE的表达及其法医学意义

龚志强;肖凤;冯琼;许小明;郑剑;   

  1. 江西医学院司法医学鉴定研究所,江西医学院司法医学鉴定研究所,江西医学院司法医学鉴定研究所,江西医学院司法医学鉴定研究所,江西医学院司法医学鉴定研究所 江西南昌330006,江西南昌330006井冈山学院医学院病理学教研室,江西吉安343000,江西南昌330006,江西南昌330006,江西南昌330006
  • 发布日期:2006-02-25 出版日期:2006-02-28

The Expression and Significance of IgE in Anaphylactic Shock Guinea-pigs

GONG ZHI-QIANG, XIAO FENG, FENG QIONG, XU XIAO-MING, ZHENG JIAN. (INSTITUTE OF FORENSIC MEDICINE, JIANGXI MEDICAL COLLEGE, NANCHANG 330006, CHINA)   

  • Online:2006-02-25 Published:2006-02-28

被引次数

37

摘要： 目的寻找法医学鉴定过敏性休克死亡的病理形态学诊断指标。方法利用组织芯片技术采用免疫组化SP法检测豚鼠过敏性休克死亡后0,6,12,24h等4个时间点的心、肝、肺、肾、脾、胃、肠、气管及扁桃体组织中的IgE的表达。结果实验组肺及气管组织IgE呈阳性表达,脾组织IgE呈弱阳性表达,以死亡即刻表达最强,且随死亡时间的延长而减弱,各时间点的显色信号强度存在显著性差异(P<0.05);实验组其它器官及对照组9个器官均无表达。结论应用免疫组化方法检测IgE在肺、气管、脾组织中的表达,可作为法医学鉴定过敏性休克死亡的病理形态学诊断指标;肺及气管组织IgE阳性强度随时间的延长而减弱,提示法医学鉴定过敏性休克死亡应尽早尸检。

关键词: 过敏性休克, IgE, 法医学鉴定, 豚鼠

Abstract: Objective To seek the pathomorphological targets for forensic expertise in anaphylactic shock. Methods The expression of IgE in hearts, lungs, livers, spleens, kidneys, gastrics, intestinals, tracheas and tonsils of anaphylactic shock guinea-pigs was observed at 0, 6, 12 h and 24 h respectively by tissue chip S-P immuno-histochemical method. Results Positive expression of IgE presented in lungs and tracheas in the test group with the peak at 0 hour and it declined as time advanced, and also there were significant differences at different times(P<0.05). Conclusion The immuno-histochemical method of detecting the expression of IgE in lungs, tracheas and spleens can be supposed to be the pathomorphological targets for forensic expertise in anaphylactic shock. The weakening of the positive expression of IgE in lungs and tracheas as the time advanced suggested that in this kind of case the autopsy should be arried out as early as possible.

Key words: anaphylactic shock, IgE, forensic expertise, guinea-pigs