法医学杂志

法医学杂志 ›› 2006, Vol. 22 ›› Issue (4): 313-314.

• 论文 • 上一篇    下一篇

血液中丙泊酚的GC-MS分析

朱友江;丁轩坤;   

  1. 湖州市人民检察院,丽水市人民检察院 浙江湖州313000,浙江丽水323000
  • 发布日期:2006-08-25 出版日期:2006-08-28

Development of a Gas Chromatography-mass Spectrometry Method for the Quantification of Propofol in Blood

ZHU YOU-JIANG1, DING XUAN-KUN2 (1.THE PEOPLE′S PROCURATORATE OF HUZHOU, HUZHOU 313000, CHINA; 2.THE PEOPLE′S PROCURATORATE OF LISHUI, LISHUI 313000, CHINA)   

  • Online:2006-08-25 Published:2006-08-28

被引次数

8

摘要： 目的应用气质联用仪(GC-MS),建立快速、灵敏地分析血液中丙泊酚的定性定量方法。方法血液1mL,加入内标,采用液液提取方法处理,GC-MS选择离子(SIM)模式测定。结果在0.1～10μg/mL范围呈良好的线性关系,相关系数R2为0.9931,最低检测限(LOD)为0.05μg/mL。结论所建立的方法简便、快速、特异性强、灵敏度高,可满足法庭毒物分析和临床毒物分析的需要。

关键词: GC-MS, 丙泊酚, 血液

Abstract: Objective A rapid and selective method has been developed for the determination of propofol in blood by gas chromatography-mass spectrometry (GC-MS). Methods The blood was extrasted with the solution of internal standard, the extracted residue was analyzed by gas chromatography-mass spectrometry using selected-ion monitoring mode. Results The linear range was 0.1-10 μg/mL. The coefficient(R2) was 0.993 1. The detection limit was 0.05 μg/mL. Conclusion The method is simple and rapid, offering superior sensitivity and selectivity for propofol. The method can be used successfully during clinical and forensic toxicology.

Key words: GC-MS, propofol, blood