法医学杂志 ›› 2016, Vol. 32 ›› Issue (4): 254-256.DOI: 10.3969/j.issn.1004-5619.2016.04.004

• 论著 • 上一篇    下一篇

应用微生物菌群鉴定阴道分泌液

邹凯南1,胡  萌2,黄江平1,周怀谷1   

  1. (1. 上海市公安局物证鉴定中心 法医物证学现场应用技术公安部重点实验室 上海市现场物证重点实验室,上海 200083; 2. 铁道警察学院,河南 郑州 450053)
  • 发布日期:2016-08-25 出版日期:2016-08-28
  • 通讯作者: 周怀谷,男,博士,主任法医师,主要从事法医DNA分析技术的应用和研究;E-mail:hgzhou803@hotmail.com
  • 作者简介:邹凯南(1983—),女,硕士,主检法医师,主要从事法医DNA分析技术的应用和研究;E-mail:kitty_zkn@126.com
  • 基金资助:

    公安部科技强警基础工作专项(2016GABJC42);上海市科学技术委员会科研计划项目(14JG0500400)

Identification of Vaginal Fluid Using Microbial Signatures

ZOU KAI-NAN1, HU MENG2, HUANG JIANG-PING1, ZHOU HUAI-GU1   

  1. (1. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. Railway Police College, Zhengzhou 450053, China)
  • Online:2016-08-25 Published:2016-08-28

摘要: 摘  要: 目的 探究特异性存在于阴道分泌液中的微生物菌群。 方法 收集阴道分泌液16份、唾液样本16份、粪便样本16份、精液样本8份、外周血样本8份、尿液样本5份及鼻腔分泌液样本4份,对卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、惰性乳杆菌和阿托波氏菌的16S rRNA基因进行扩增,PCR产物采用3130xl型遗传分析仪进行检测。 结果 卷曲乳杆菌、加氏乳杆菌、詹氏乳杆菌、惰性乳杆菌和阿托波氏菌在阴道分泌液中的检出份数分别为15、5、8、14和3份。卷曲乳杆菌和詹氏乳杆菌特异性存在于阴道分泌液中。 结论 检测卷曲乳杆菌和詹氏乳杆菌对鉴定阴道分泌液具有潜在的应用价值。

关键词: 法医遗传学, 微生物学, 遗传标记, 阴道分泌液

Abstract: Abstract: Objective To investigate the specific microbial signatures in vaginal fluid. Methods Vaginal fluid (16 samples), saliva (16 samples), feces (16 samples), semen (8 samples), peripheral blood (8 samples), urine (5 samples), and nasal secretion (4 samples) were collected respectively. The 16S rRNA genes of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were amplified. PCR production was detected via a 3130xl Genetic Analyzer. Results The detected number of Lactobacillus crispatus, Lactobacillus gasseri, Lactobacillus jensenii, Lactobacillus iners, and Atopobium vaginae were 15, 5, 8, 14, and 3 in all vaginal fluid samples, respectively. Lactobacillus crispatus and Lactobacillus jensenii existed specifically in vaginal fluid. Conclusion There is a potential application value to detect Lactobacillus crispatus and Lactobacillus jensenii for the identification of vaginal fluid.

Key words: forensic genetics, microbiology, genetic markers, vaginal fluid

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