法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (5): 356-362.DOI: 10.3969/j.issn.1004-5619.2016.05.010

• 技术与应用 • 上一篇    下一篇

湖南汉族人群21个STR基因座的遗传多态性（英文）

邹  鹰1，郭娟娟1，李情鹏2，左道宏2，刘金山3，郭亚东1，闫  杰1，扎拉嘎白乙拉1，蔡继峰1，兰玲梅1   

  1. （1. 中南大学基础医学院法医学系，湖南 长沙 410013； 2. 中南大学湘雅医学院，湖南 长沙 410013； 3. 内蒙古民族大学附属医院内科，内蒙古 通辽 028000）
  • 发布日期:2016-10-25 出版日期:2016-10-28

Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population

ZOU YING1, GUO JUAN-JUAN1, LI QING-PENG2, ZUO DAO-HONG2, LIU JIN-SHAN3, GUO YA-DONG1, YAN JIE1, ZHA LAGABAIYILA1, CAI JI-FENG1, LAN LING-MEI1   

  1. （1. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China; 2. Xiangya School of Medicine, Central South University, Changsha 410013, China; 3. Department of Internal Medicine, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China）
  • Online:2016-10-25 Published:2016-10-28
  • Contact: LAN Ling-mei, M.S., experimentalist, major in forensic genetics; E-mail:lanlingmei0731@163.com
  • About author:ZOU Ying, M.S., experimentalist, major in forensic genetics; E-mail: zouhawk@163.com
  • Supported by:

    the Natural Science Foundation for the Youth （No. 81302621） and the Fundamental Research Funds for the Central Universities of Central South University （2015zzts276）

摘要： 目的 调查湖南地区汉族人群21个STR基因座（D3S1358、D13S317、D7S820、D16S539、Penta E、D2S441、TPOX、TH01、D2S1338、CSF1PO、Penta D、D10S1248、D19S433、vWA、D21S11、D18S51、D6S1043、D8S1179、D5S818、D12S391和FGA）的遗传多态性。 方法 共采集560例湖南汉族健康无关个体血液样本，使用Chelex-100法提取DNA，应用AGCU EX22试剂盒及9700 PCR扩增仪进行复合扩增，扩增产物使用310遗传分析仪进行分离分析。 结果 共发现248个等位基因，等位基因频率分布在0.001~0.518。除Penta E（P=0.023）外，其余基因座的基因型分布均符合Hardy-Weinberg平衡。21个基因座的累积个人识别率、累积非父排除率、累积匹配率分别为0.999 999 999 999 999 999 999 999 8、0.999 999 998和1.36×10-25。 结论 21个STR基因座在湖南汉族人群中呈高度多态性。本研究可为法医学个人识别及亲子鉴定提供有价值的数据及理论基础。

关键词: 法医遗传学, 多态现象, 遗传, 短串联重复序列, 湖南, 汉族

Abstract: Objective To investigate the genetic polymorphisms of 21 short tandem repeat （STR） loci （D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA）. Methods A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer. Results A total of 248 alleles were observed, the allelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium except Penta E （P=0.023）. The combined power of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10-25, respectively. Conclusion The 21 STR loci show high polymorphisms in the Han population, which can provide valuable data and a theoretical basis for forensic individual identification and paternity testing.

Key words: forensic genetics, polymorphism, genetic, short tandem repeat sequences, Hunan, Han nationality

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