法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (2): 168-170.DOI: 10.3969/j.issn.1004-5619.2017.02.013

• 技术与应用 • 上一篇    下一篇

MPure-12全自动核酸纯化仪与Chelex-100法的比较

盛  翔1，2，李  敏2，3，王亚丽2，4，陈玉玲2，5，林  源2，赵珍敏2，阙庭志2   

  1. （1. 苏州大学医学部法医学系，江苏 苏州 215123； 2. 司法部司法鉴定科学技术研究所 上海市法医学重点实验室 上海市司法鉴定专业技术服务平台，上海 200063； 3. 温州医科大学基础医学院，浙江 温州 325000； 4. 内蒙古医科大学基础医学院法医学，内蒙古 呼和浩特 010020； 5. 华东理工大学生物工程学院 生物反应器工程国家重点实验室，上海 200237）
  • 发布日期:2017-04-25 出版日期:2017-04-28
  • 通讯作者: 阙庭志，男，副主任法医师，主要从事法医物证学研究；E-mail：quetz@ssfjd.cn
  • 作者简介:盛翔（1991—），女，硕士研究生，主要从事法医遗传学研究；E-mail：dreamer_sx0926@163.com
  • 基金资助:

    十三五国家重点研发计划项目(2016YFC0800703)；中央级科研院所公益资助项目（GY2014G-4，GY2017D-2）；上海市法医学重点实验室资助项目（17DZ2273200）；上海市司法鉴定专业技术服务平台资助项目（16DZ2290900）

Comparison of MPure-12 Automatic Nucleic Acid Purification and Chelex-100 Method

SHENG XIANG1,2, LI MIN2,3, WANG YA-LI2,4, CHEN YU-LING2,5, LIN YUAN2, ZHAO ZHEN-MIN2, QUE TING-ZHI2   

  1. （1. Department of Forensic Medicine, Medical College of Soochow University, Suzhou 215123, China; 2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Institute of Forensic Science, Ministry of Justice, PRC, Shanghai 200063, China; 3. School of Basic Medical Sciences, Wenzhou Medical University, Wenzhou 325000, China; 4. Department of Forensic Medicine, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010020, China; 5. School of Biotechnology, East China University of Science and Technology, State Key Laboratory of Bioreactor Engineering, Shanghai 200237, China）
  • Online:2017-04-25 Published:2017-04-28

摘要： 目的 通过对DNA含量不同的血痕和多种类型生物学检材进行DNA提取和STR分型检测，探讨MPure-12全自动核酸纯化仪（MPure-12法）在DNA提取中的法医学应用价值。 方法 收集血痕、精斑、唾液等9种类型的生物学检材，应用MPure-12法和传统Chelex-100法提取DNA，经过PCR扩增和电泳，获取STR分型图谱。 结果 MPure-12法对发根、口香糖、烟蒂、肌肉组织、唾液斑、血痕、精斑这些检材能够成功分型，唾液出现了个别等位基因丢失现象，接触拭子分型较差。Chelex-100法对血量为20 μL、15 μL、10 μL、5 μL、1 μL制成的血痕均有完整分型结果，MPure-12法在血量为1 μL时出现等位基因丢失现象。 结论 MPure-12法适用于一定浓度血样的检验，而对于微量血样，Chelex-100法提取DNA的效果可能更优。仪器应用于DNA提取具有操作简单、快速、提取效率高，分型成功率高，减少人为污染等优势。

关键词: 法医遗传学, 提取法, 自动化, 实验室, MPure-12全自动核酸纯化仪, Chelex-100法

Abstract: Objective To explore the forensic application value of MPure-12 automatic nucleic acid purification （MPure-12 Method） for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. Methods Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles. Results The samples such as hair root, chutty, butt, muscular tissue, saliva stain, bloodstain and semen stain were typed successfully by MPure-12 method. Partial alleles were lacked in the samples of saliva, and the genotyping of contact swabs was unsatisfactory. Additional, all of the bloodstains （20 μL, 15 μL, 10 μL, 5 μL, 1 μL） showed good typing results using Chelex-100 method. But the loss of alleles occurred in 1 μL blood volume by MPure-12 method. Conclusion MPure-12 method is suitable for DNA extraction of a certain concentration blood samples．Chelex-100 method may be better for the extraction of trace blood samples．This instrument used in nucleic acid extraction has the advantages of simplicity of operator, rapidity, high extraction efficiency, high rate of reportable STR-profiles and lower man-made pollution.

Key words: forensic genetics, extraction, automation, laboratory, MPure-12 automatic nucleic acid purification, Chelex-100 method

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