法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (3): 273-279.DOI: 10.12116/j.issn.1004-5619.2019.03.002

• 论著 • 上一篇    下一篇

人脑皮质挫伤后不同细胞Nrf2的表达

郭相伸1，2， 温书恒1，2， 董雯雯1，2， 李炳譞1，3， 陈子原1，2， 王林林1，2， 官大威1，2， 赵锐1，2   

  1. 1. 中国医科大学法医学院法医司法鉴定中心，辽宁 沈阳 110122； 2. 智慧检务创新研究院 智慧司法鉴定联合实验室，辽宁 沈阳 110122； 3. 中国刑事警察学院，辽宁 沈阳 110035
  • 发布日期:2019-06-25 出版日期:2019-06-28
  • 通讯作者: 赵锐，男，教授，博士研究生导师，主要从事法医病理学教学、科研和鉴定；E-mail：rzhao@cmu.edu.cn
  • 作者简介:郭相伸（1992—），男，硕士研究生，主要从事法医病理学教学、科研和鉴定；E-mail：137190485@qq.com
  • 基金资助:
    国家自然科学基金资助项目（81772023，81671862，81871529，81801874）；“十三五”国家重点研发计划资助项目（2018 TFC0807204）

Expression of Nrf2 in Different Cells after Human Cerebral Cortex Contusion

GUO Xiang-shen1,2, WEN Shu-heng1,2, DONG Wen-wen1,2, LI Bing-xuan1,3, CHEN Zi-yuan1,2, WANG Lin-lin1,2, GUAN Da-wei1,2, ZHAO Rui1,2   

  1. 1. Center of Medico-legal Investigation, School of Forensic Medicine, China Medical University, Shenyang 110122, China; 2. Collaborative Laboratory of Intelligentized Forensic Science, Shenyang 110122, China; 3. Criminal Investigation Police University of China, Shenyang 110035, China
  • Online:2019-06-25 Published:2019-06-28

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摘要： 目的 观察核因子E2相关因子2（nuclear factor-erythroid derived 2-related factors，Nrf2）在人脑皮质挫伤后不同时间段不同细胞内的表达变化，并探讨其在脑损伤时间推断中的应用。 方法 选取36例人脑组织，其中6例为对照，30例为不同时间段的脑皮质挫伤，分成伤后0~1 h、3~6 h、1~3 d、5~7 d、10~14 d组，每组6例。石蜡切片，HE染色，观察损伤后的病理形态学变化，应用免疫荧光双染色法检测Nrf2在神经元、星形胶质细胞和小胶质细胞中的表达情况，计数阳性细胞数并进行统计分析。 结果 外伤后1~3 d神经元出现死亡，Nrf2在伤后的神经元内表达增高，阳性细胞率在1~3 d达到高峰。星形胶质细胞伤后1~3 d活化，5~7 d达到高峰，10~14 d软化灶形成。伤后小鼠抗人胶质纤维酸性蛋白（glial fibrillary acidic protein，GFAP）阳性细胞逐渐增多，伤后5~7 d达高峰，而Nrf2在GFAP阳性细胞比例相对稳定。外伤后，小鼠抗人离子钙接头蛋白分子1（ionized calcium-binding adapter molecule 1，IBA1）阳性细胞逐渐增多、激活，Nrf2在IBA1阳性细胞中的表达比例逐渐增高，伤后5~7 d达到高峰，之后开始下降。 结论 Nrf2在不同细胞内的表达参与不同细胞损伤后的生物学功能，其在单种细胞的动态表达存在时间规律性，有望为人脑挫伤后损伤经过时间推断提供新的参考指标。

关键词: 法医病理学, 颅脑损伤, 核因子E2相关因子2, 损伤时间推断

Abstract: Objective To observe the expression changes of nuclear factor-erythroid derived 2-related factors （Nrf2） in different cells at different time points after human cerebral cortex contusion, and to discuss its application in brain wound age estimation. Methods Thirty-six human brain tissues were selected, of which 6 were for control and 30 were cortical contusion at different time points post-injury, which were divided into 0-1 h, 3-6 h, 1-3 d, 5-7 d, and 10-14 d post-injury groups, with 6 cases in each group. Based on paraffin embedded sections, HE staining was used to observe the morphological changes post-injury, and double immunofluorescence staining was used to detect the expression of Nrf2 in neurons, astrocytes, and microglia. The number of positive cells was counted and statistical analysis was made. Results The number of neurons decreased 1-3 d post-injury. The expression of Nrf2 cells in neurons increased after injury, and the rate of positive cells peaked at 1-3 d post-injury. Glial cells were activated 1-3 d post-injury, and the activation peaked at 5-7 d post-injury. The cerebromalacia began to form at 10-14 d post-injury. Glial fibrillary acidic protein （GFAP） positive cells in mice increased gradually after injury and peaked at 5-7 d post-injury, while the proportion of Nrf2 in GFAP positive cells was relatively stable. After injury, ionized calcium-binding adapter molecule 1 （IBA1） positive cells increased and activated gradually. The expression proportion of Nrf2 in IBA1 positive cells increased gradually, reached its peak at 5-7 d post-injury, and then decreased. Conclusion The expression of Nrf2 in different cells involves in the biological function of different cells post-injury, and the dynamic expression of single cells has a time-dependent pattern. This may provide a new reference index for the wound age estimation of brain contusion in human.

Key words: forensic pathology, craniocerebral trauma, nuclear factor erythroid 2-related factor 2, wound age estimation