法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (3): 280-284.DOI: 10.12116/j.issn.1004-5619.2019.03.003

• 论著 • 上一篇    下一篇

小鼠骨骼肌挫伤愈合过程中神经肌肉接头降解及再生与损伤时间的关系

孙英富1，2， 张孟周1，2， 王帅1，2， 董雯雯1，2， 陈京伟1，2， 郭相伸1，2， 温书恒1，2， 赵锐1，2， 官大威1，2   

  1. 1. 中国医科大学法医学院 法医司法鉴定中心，辽宁 沈阳 110122； 2. 智慧检务创新研究院 智慧司法鉴定联合实验室，辽宁 沈阳 110122
  • 发布日期:2019-06-25 出版日期:2019-06-28
  • 通讯作者: 官大威，男，博士，教授，博士研究生导师，主要从事器官组织损伤修复分子生物学机制与损伤时间推断研究；E-mail：dwguan@cmu.edu.cn
  • 作者简介:孙英富（1992—），男，硕士研究生，主要从事骨骼肌损伤修复研究；E-mail：444795519@qq.com
  • 基金资助:
    国家自然科学基金资助项目（81273342，81671862，81772023，81871529，81801874）；国家重点研发计划子课题资助项目（2018YFC0807204）；中国工程院重大咨询研究子课题资助项目（2016-XZ-21-03）

Relationship between the Degeneration and Regeneration of Neuromuscular Junction and Wound Age during the Repair of Mouse Skeletal Muscle Contusion

SUN Ying-fu1,2, ZHANG Meng-zhou1,2, WANG Shuai1,2, DONG Wen-wen1,2, CHEN Jing-wei1,2, GUO Xiang-shen1,2, WEN Shu-heng1,2, ZHAO Rui1,2, GUAN Da-wei1,2   

  1. 1. Center of Medico-legal Investigation, School of Forensic Medicine, China Medical University, Shenyang 110122, China; 2. Collaborative Laboratory of Intelligentized Forensic Science, Shenyang 110122, China
  • Online:2019-06-25 Published:2019-06-28

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摘要： 目的 研究小鼠骨骼肌挫伤愈合过程中神经肌肉接头（neuromuscular junction，NMJ）降解及再生的形态学变化，探讨NMJ降解及再生与损伤时间的相关性。 方法 将50只健康成年雄性小鼠随机分为10组，其中9组为实验组，1组为对照组。应用免疫荧光染色技术，以高分子量神经丝蛋白（neurofilament protein-H，NF-H）标记神经丝，突触囊泡蛋白（synaptophysin，Syn）标记突触前膜，乙酰胆碱受体（acetylcholine receptor，AChR）标记突触后膜，检测小鼠骨骼肌挫伤后不同时间点NMJ再生的形态学变化。 结果 骨骼肌挫伤后挫伤交界区NMJ突触前膜及神经丝开始降解，3 d左右降解完全，之后逐渐再生，至21 d左右再生大致完成，基本达到对照组水平。突触前膜和后膜数量之比呈现先下降后上升、最终与对照组水平相当的趋势。 结论 小鼠骨骼肌挫伤修复过程中，挫伤交界区NMJ的形态学变化与损伤时间具有相关性，有望成为法医学骨骼肌损伤时间推断的生物学指标之一。

关键词: 法医病理学, 骨骼肌, 挫伤, 神经肌肉接头, 损伤时间推断, 小鼠

Abstract: Objective To investigate the morphological changes in the degeneration and regeneration of neuromuscular junctions （NMJ） during the repair of mouse skeletal muscle contusion and discuss the correlation between the degeneration and regeneration of NMJ and wound age. Methods A total of 50 healthy adult male mice were randomly divided into 10 groups, including 9 experimental groups and 1 control group. Immunofluorescent staining was applied, and neurofilament was marked with neurofilament protein-H （NF-H）, presynaptic membrane was marked with synaptophysin （Syn）, presynaptic membrane was marked with acetylcholine receptor （AChR）. Morphological changes of NMJ regeneration at different time points after mouse skeletal muscle contusion were detected. Results The neurofilament and presynaptic membrane of NMJ at the junction of contusion zones began to degrade after contusion, and completed degradation at about 3 d post-injury. Then they gradually regenerated, roughly completing the regeneration at about 21 d and basically reaching the control group level. The ratio of presynaptic membrane quantity to presynaptic membrane quantity showed a trend of decreasing then rising and finally reaching the control level. Conclusion During the repair of mouse skeletal muscle contusion, the morphological changes and wound age of the NMJ at the junction of contusion zones have a close correlation, which is expected to be one of the biological indicators for forensic skeletal muscle wound age estimation.

Key words: forensic pathology, skeletal muscle, contusion, neuromuscular junction, wound age determination, mice