Efficiency Analysis of Uncle-Nephew Relationship Identification by Increasing STR Markers and Adding Reference Samples

doi:10.12116/j.issn.1004-5619.2022.520407

Abstract

Abstract:

Objective To estimate the system efficiency of uncle-nephew relationship identification by increasing STR markers and adding reference samples based on the test results of simulated data and real samples, so as to provide references for selecting the appropriate number of STRs and reference samples for uncle-nephew relationship identification. Methods Five common models of uncle-nephew relationship identification were constructed by adding different reference samples. In each model, the likelihood ratio （LR） for 10 000 pairs of uncle-nephew relationships and 10 000 pairs of unrelated individuals were simulated by detecting 19, 39 or 55 STRs, and the system efficiency at different thresholds was simulated. The samples of the Han population in Zhejiang were collected, and 55 autosomal STRs were obtained by using SiFaSTR^TM 23plex kit, Goldeneye^? DNA ID 22NC kit and AGCU 21+1 PCR amplification kit. When 19, 39 and 55 STRs were detected, the LR of each model and system efficiency under different thresholds were calculated and compared with the simulation results. Results Under the same detection system, the calculated results of simulated data and corresponding true samples were basically consistent. In the same model, there was a positive correlation between the system efficiency of uncle-nephew relationship identification and the number of STRs detected. Moreover, the system efficiency of introducing relatives was higher than identifying only two individuals. The order of preference for the introduction of relatives was the full sibling （or mother） of the uncle and the full sibling （or mother） of the nephew. Conclusion The system efficiency of uncle-nephew relationship identification could be improved by increasing the number of STRs and introducing known relatives, which would provide the basis for selecting the most appropriate detection system and reference individuals in actual cases.

Key words: forensic genetics, uncle-nephew relationship identification, STR, autosome, system efficiency

CLC Number:

DF795.2

Qian-qian KONG, Li-qin CHEN, Cheng-tao LI, Xi-ling LIU. Efficiency Analysis of Uncle-Nephew Relationship Identification by Increasing STR Markers and Adding Reference Samples[J]. Journal of Forensic Medicine, 2022, 38(3): 360-366.

Figures/Tables 4

References 25

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来源	（AB）	（AB）-UR	（ABC）A	（ABC）A-UR	（ABE）A	（ABE）A-UR	（ABD）B	（ABD）B-UR	（ABM）B	（ABM）B-UR
模拟数据
55 STR	3.970 3	-3.539 8	6.240 5	-7.856 0	6.261 6	-6.756 5	6.229 0	-5.321 1	6.182 7	-5.196 9
39 STR	3.091 3	-2.755 0	4.951 5	-6.433 6	4.951 4	-5.312 8	4.887 7	-4.186 5	4.895 6	-4.084 6
19 STR	1.516 3	-1.339 3	2.449 8	-3.184 9	2.439 1	-2.565 4	2.410 0	-2.012 2	2.380 6	-1.960 0
真实样本
55 STR	3.012 0	-3.787 2	3.970 8	-9.090 1	5.852 6	-7.073 7	5.048 8	-6.119 9	4.648 8	-5.700 8
39 STR	2.555 0	-2.985 5	3.883 6	-7.255 8	4.652 1	-5.492 6	3.998 9	-4.717 6	4.067 1	-4.418 3
19 STR	0.994 9	-1.467 6	1.620 5	-3.915 1	1.977 8	-2.723 1	1.596 2	-2.447 2	1.759 8	-2.178 1

来源	（AB）	（AB）-UR	（ABC）A	（ABC）A-UR	（ABE）A	（ABE）A-UR	（ABD）B	（ABD）B-UR	（ABM）B	（ABM）B-UR
模拟数据
55 STR	3.970 3	-3.539 8	6.240 5	-7.856 0	6.261 6	-6.756 5	6.229 0	-5.321 1	6.182 7	-5.196 9
39 STR	3.091 3	-2.755 0	4.951 5	-6.433 6	4.951 4	-5.312 8	4.887 7	-4.186 5	4.895 6	-4.084 6
19 STR	1.516 3	-1.339 3	2.449 8	-3.184 9	2.439 1	-2.565 4	2.410 0	-2.012 2	2.380 6	-1.960 0
真实样本
55 STR	3.012 0	-3.787 2	3.970 8	-9.090 1	5.852 6	-7.073 7	5.048 8	-6.119 9	4.648 8	-5.700 8
39 STR	2.555 0	-2.985 5	3.883 6	-7.255 8	4.652 1	-5.492 6	3.998 9	-4.717 6	4.067 1	-4.418 3
19 STR	0.994 9	-1.467 6	1.620 5	-3.915 1	1.977 8	-2.723 1	1.596 2	-2.447 2	1.759 8	-2.178 1

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