Journal of Forensic Medicine ›› 2024, Vol. 40 ›› Issue (2): 211-216.DOI: 10.12116/j.issn.1004-5619.2023.430407
Received:2023-04-14
Online:2024-06-07
Published:2024-04-25
CLC Number:
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.fyxzz.cn/EN/10.12116/j.issn.1004-5619.2023.430407
Fig. 1 Extensive petechia on the trunk and limbs
Fig. 2 Diffuse edema of lung tissue and hyaline thrombosisin pulmonary interstitial vessels （HE×100）
| 样本 | 检出的病原体 |
|---|---|
| 尿道口拭子 | E. bugandensis、阴道加德纳菌、咽峡炎链球菌 |
| 腋窝拭子 | 棒状杆菌属菌、表皮葡萄球菌 |
| 扁桃体 | 中间链球菌 |
| 肺 | - |
| 小肠 | 粪肠球菌 |
| 膀胱 | E. bugandensis、粪肠球菌 |
| 左手背皮下软组织 | - |
| 右手背皮下软组织 | - |
| 血液 | E. bugandensis |
| 复方氨基酸注射液 | E. bugandensis |
| 氯化钠溶液 | - |
Tab. 1 mNGS detection results ofpathogens in each sample
| 样本 | 检出的病原体 |
|---|---|
| 尿道口拭子 | E. bugandensis、阴道加德纳菌、咽峡炎链球菌 |
| 腋窝拭子 | 棒状杆菌属菌、表皮葡萄球菌 |
| 扁桃体 | 中间链球菌 |
| 肺 | - |
| 小肠 | 粪肠球菌 |
| 膀胱 | E. bugandensis、粪肠球菌 |
| 左手背皮下软组织 | - |
| 右手背皮下软组织 | - |
| 血液 | E. bugandensis |
| 复方氨基酸注射液 | E. bugandensis |
| 氯化钠溶液 | - |
Fig. 3 Sequencing depth and coverage of E. bugandensis in each sample
Fig. 4 E. bugandensis in spleen and liver tissues（detected by FISH， 1 000×）
| 1 | PRUDENT E， RAOULT D. Fluorescence in situ hybridization， a complementary molecular tool for the clinical diagnosis of infectious diseases by intracellular and fastidious bacteria[J]. FEMS Microbiol Rev，2019，43（1）：88-107. doi：10.1093/femsre/fuy040 . |
| 2 | SINGER M， DEUTSCHMAN C S， SEYMOUR C W， et al. The third international consensus definitions for sepsis and septic shock （sepsis-3）[J]. JAMA，2016，315（8）：801-810. doi：10.1001/jama.2016.0287 . |
| 3 | RUDD K E， JOHNSON S C， AGESA K M， et al. Global， regional， and national sepsis incidence and mortality， 1990—2017： Analysis for the Global Burden of Disease Study[J]. Lancet，2020，395（10219）：200-211. doi：10.1016/S0140-6736（19）32989-7 . |
| 4 | PETSCH D， ANSPACH F B. Endotoxin removal from protein solutions[J]. J Biotechnol，2000，76（2/3）：97-119. doi：10.1016/s0168-1656（99）00185-6 . |
| 5 | ŚMIECHOWICZ J. The rationale and current status of endotoxin adsorption in the treatment of septic shock[J]. J Clin Med，2022，11（3）：619. doi：10.3390/jcm11030619 . |
| 6 | TANI V M， ASSIS-MENDONÇA G R， SILVA T B DA， et al. Microvascular thrombosis in sepsis： An autopsy study[J]. Thromb Res，2017，156：23-25. doi：10.1016/j.thromres.2017.05.031 . |
| 7 | YANG Y， XIE J， GUO F， et al. Combination of C-reactive protein， procalcitonin and sepsis-related organ failure score for the diagnosis of sepsis in critical patients[J]. Ann Intensive Care，2016，6（1）：51. doi：10.1186/s13613-016-0153-5 . |
| 8 | MSHANA S E， GERWING L， MINDE M， et al. Outbreak of a novel Enterobacter sp. carrying blaCTX-M-15 in a neonatal unit of a tertiary care hospital in Tanzania[J]. Int J Antimicrob Agents，2011，38（3）：265-269. doi：10.1016/j.ijantimicag.2011.05.009 . |
| 9 | FALGENHAUER J， IMIRZALIOGLU C， FAL-GENHAUER L， et al. Whole-genome sequences of clinical Enterobacter bugandensis isolates from Germany[J]. Microbiol Resour Announc，2019，8（29）：e00465-e00419. doi：10.1128/MRA.00465-19 . |
| 10 | PATI N B， DOIJAD S P， SCHULTZE T， et al. Enterobacter bugandensis： A novel enterobacterial species associated with severe clinical infection[J]. Sci Rep，2018，8（1）：5392. doi：10.1038/s41598-018-23069-z . |
| 11 | SALLEM R BEN， ARFAOUI A， NAJJARI A， et al. First report of IMI-2-producing Enterobacter bugandensis and CTX-M-55-producing Escherichia coli isolated from healthy volunteers in Tunisia[J]. Antibiotics （Basel），2023，12（1）：116. doi：10.3390/antibiotics12010116 . |
| 12 | GU W， MILLER S， CHIU C Y. Clinical metagenomic next-generation sequencing for pathogen detection[J]. Annu Rev Pathol，2019，14：319-338. doi：10.1146/annurev-pathmechdis-012418-012751 . |
| 13 | CALISTRI A， PALÙ G. Editorial commentary： Unbiased next-generation sequencing and new pathogen discovery： Undeniable advantages and still-existing drawbacks[J]. Clin Infect Dis，2015，60（6）：889-891. doi：10.1093/cid/ciu913 . |
| 14 | GRUMAZ C， HOFFMANN A， VAINSHTEIN Y， et al. Rapid next-generation sequencing-based diagnostics of bacteremia in septic patients[J]. J Mol Diagn，2020，22（3）：405-418. doi：10.1016/j.jmoldx.2019.12.006 . |
| 15 | LONG Y， ZHANG Y， GONG Y， et al. Diagnosis of sepsis with cell-free DNA by next-generation sequencing technology in ICU patients[J]. Arch Med Res，2016，47（5）：365-371. doi：10.1016/j.arcmed.2016.08.004 . |
| 16 | SHERIDAN C. COVID-19 spurs wave of innovative diagnostics[J]. Nat Biotechnol，2020，38（7）：769-772. doi：10.1038/s41587-020-0597-x . |
| 17 | KUIPER I. Microbial forensics： Next-generation sequencing as catalyst： The use of new sequencing technologies to analyze whole microbial communities could become a powerful tool for forensic and criminal investigations[J]. EMBO Rep，2016，17（8）：1085-1087. doi：10.15252/embr.201642794 . |
| 18 | SCHMEDES S E， SAJANTILA A， BUDOWLE B. Expansion of microbial forensics[J]. J Clin Microbiol，2016，54（8）：1964-1974. doi：10.1128/JCM.00046-16 . |
| 19 | YU Z， XIE Q， ZHAO Y， et al. NGS plus bacterial culture： A more accurate method for diagnosing forensic-related nosocomial infections[J]. Leg Med （Tokyo），2021，52：101910. doi：10.1016/j.legalmed.2021.101910 . |
| 20 | GUO J， FU X， LIAO H， et al. Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing[J]. Sci Rep，2016，6：24197. doi：10.1038/srep24197 . |
| 21 | HYDE E R， HAARMANN D P， LYNNE A M， et al. The living dead： Bacterial community structure of a cadaver at the onset and end of the bloat stage of decomposition[J]. PLoS One，2013，8（10）：e77733. doi：10.1371/journal.pone.0077733 . |
| 22 | RIEDEL S. The value of postmortem microbiology cultures[J]. J Clin Microbiol，2014，52（4）：1028-1033. doi：10.1128/JCM.03102-13 . |
| 23 | SIMNER P J， MILLER S， CARROLL K C. Understanding the promises and hurdles of metagenomic next-generation sequencing as a diagnostic tool for infectious diseases[J]. Clin Infect Dis，2018，66（5）：778-788. doi：10.1093/cid/cix881 . |
| 24 | GOSIEWSKI T， LUDWIG-GALEZOWSKA A H， HUMINSKA K， et al. Comprehensive detection and identification of bacterial DNA in the blood of patients with sepsis and healthy volunteers using next-generation sequencing method — The observation of DNAemia[J]. Eur J Clin Microbiol Infect Dis，2017，36（2）：329-336. doi：10.1007/s10096-016-2805-7 . |
| 25 | 中华医学会检验医学分会临床微生物学组，中华医学会微生物学与免疫学分会临床微生物学组，中国医疗保健国际交流促进会临床微生物与感染分会. 宏基因组高通量测序技术应用于感染性疾病病原检测中国专家共识[J].中华检验医学杂志，2021，44（2）：107-120. doi：10.3760/cma.j.cn114452-20201026-00794 . |
| Clinical Microbiology Group of Chinese Society of Laboratory Medicine， Clinical Microbiology Group of Chinese Society of Microbiology and Immunology， Society of Clinical Microbiology and Infection of China International Exchange and Promotion Association for Medical and Healthcare. Chinese expert consensus on metagenomics next-generation sequencing application on pathogen detection of infectious diseases[J]. Zhonghua Jianyan Yixue Zazhi，2021，44（2）：107-120. | |
| 26 | 宏基因组学测序技术在中重症感染中的临床应用共识专家组，中国研究型医院学会脓毒症与休克专业委员会，中国微生物学会微生物毒素专业委员会，等. 宏基因组学测序技术在中重症感染中的临床应用专家共识（第一版）[J].中华危重病急救医学，2020，32（5）：531-536. doi：10.3760/cma.j.cn121430-20200228-00095 . |
| Consensus Group of Experts on Application of Metagenomic Next Generation Sequencing in the Pathogen Diagnosis in Clinical Moderate and Severe Infections， Professional Committee of Sepsis and Shock， Chinese Research Hospital Association， Professional Committee of Microbial Toxins， Chinese Society for Microbiology， et al. Expert consensus for the application of metagenomic next generation sequencing in the pathogen diagnosis in clinical moderate and severe infections （first edition）[J]. Zhonghua Weizhongbing Jijiu Yixue，2020，32（5）：531-536. | |
| 27 | 中华医学会检验医学分会. 高通量宏基因组测序技术检测病原微生物的临床应用规范化专家共识[J].中华检验医学杂志，2020，43（12）：1181-1195. doi：10.3760/cma.j.cn114452-20200903-00704 . |
| Chinese Society of Laboratory Medicine. Expert consensus on clinical standardized application of metagenomics next-generation sequencing for detection of pathogenic microorganisms[J]. Zhonghua Jianyan Yi-xue Zazhi，2020，43（12）：1181-1195. | |
| 28 | MALLA M A， DUBEY A， KUMAR A， et al. Exploring the human microbiome： The potential future role of next-generation sequencing in disease diagnosis and treatment[J]. Front Immunol，2018，9：2868. doi：10.3389/fimmu.2018.02868 . |
| [1] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 208-210. |
| [2] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 205-207. |
| [3] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 202-204. |
| [4] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 199-201. |
| [5] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 196-199. |
| [6] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 192-195. |
| [7] | Jia-hao LIANG, Ming CHENG, Xiao-jun LU, Yan-hua SHI, Yun SUN, Qing-lin GUAN, Tao WANG, Meng HU, Ke-ming YUN, Hai-yan CUI. Postmortem Diffusion of Aconitum Alkaloids and Their Metabolites in Rabbits [J]. Journal of Forensic Medicine, 2024, 40(2): 186-191. |
| [8] | Juan-juan WU, Jun-jie HUANG, Yu ZHANG, Jia-ying ZHUO, Gang CHEN, Shu-han YANG, Yun-qi ZHAO, Yan-yan FAN. Time-Dependent Sequential Changes of IL-10 and TGF-β1 in Mice with Deep Vein Thrombosis [J]. Journal of Forensic Medicine, 2024, 40(2): 179-185. |
| [9] | Shang-heng CHEN, Sheng-zhong DONG, Zhi-min WANG, Guang-hui HONG, Xing YE, Zi-jie LIN, Jun-yi LIN, Jie-qing JIANG, Shou-yu WANG, Han-cheng LIN, Yi-wen SHEN. Biomarkers Screening and Mechanisms Analysis of the Restraint Stress-Induced Myocardial Injury in Hyperlipidemia ApoE-/- Mice [J]. Journal of Forensic Medicine, 2024, 40(2): 172-178. |
| [10] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 168-171. |
| [11] | . [J]. Journal of Forensic Medicine, 2024, 40(2): 164-167. |
| [12] | Huai-han ZHANG, Yong-jie CAO, Ji ZHANG, Jian XIONG, Ji-wei MA, Xiao-tong YANG, Ping HUANG, Yong-gang MA. Adults Ischium Age Estimation Based on Deep Learning and 3D CT Reconstruction [J]. Journal of Forensic Medicine, 2024, 40(2): 154-163. |
| [13] | De-min HUO, Kai-jun MA, Jing-lan XU, Xu SONG, Xiao-yan MAO, Xia LIU, Kai-fang ZHAO, Jian ZHANG, Meng DU. Determining Whether an Individual is 18 Years or Older Based on the Third Molar Root Pulp Visibility in East China [J]. Journal of Forensic Medicine, 2024, 40(2): 149-153. |
| [14] | Jia-xuan HAN, Shi-hui SHEN, Yi-wen WU, Xiao-dan SUN, Tian-nan CHEN, Jiang TAO. Adolescents and Children Age Estimation Using Machine Learning Based on Pulp and Tooth Volumes on CBCT Images [J]. Journal of Forensic Medicine, 2024, 40(2): 143-148. |
| [15] | Yu-xin GUO, Wen-qing BU, Yu TANG, Di WU, Hui YANG, Hao-tian MENG, Yu-cheng GUO. Dental Age Estimation in Northern Chinese Han Children and Adolescents Using Demirjian’s Method Combined with Machine Learning Algorithms [J]. Journal of Forensic Medicine, 2024, 40(2): 135-142. |
| Viewed | ||||||
|
Full text |
|
|||||
|
Abstract |
|
|||||
