Abstract: Objective To analyze the variations of glycerol-3-phosphate dehydrogenase 1 like gene （GPD1-L） and address the association with sudden manhood death syndrome （SMDS）. Methods The genomic DNA was extracted from blood samples of the SMDS group and the normal control group. The exons, exon-intron boundaries and 3′-UTRs of coding region of GPD1-L were PCR amplified and DNA sequenced directly to confirm the types of variations. The genotype frequency and allele frequency were analyzed statistically. Results There were two variants in the SMDS group, c.465C＞T and c.*18G＞T, the latter existed certain degree difference of genotype distribution and allele frequency between the SMDS group and the control group, but there was no statistically significant（P＞0.05）. Conclusion The relation between gene mutation of GPD1-L and the occurrence of Chinese SMDS deserves a further research.

Key words: forensic genetics, sudden manhood death syndrome, glycerol-3-phosphate dehydrogenase 1 like gene