Abstract: Objective To investigate the genetic polymorphisms of 19 STR Loci in Shandong Han population in order to provide the genetic data for paternity testing. Methods The genotypes of 205 unrelated individuals in Shandong Han population were typed by GoldeneyeTM 20A kit to get the allele frequencies and population genetic parameters of 19 STR loci. Four kits, IdentifilerTM kit, SinoFilerTM kit, PowerPlex 16 kit, and GoldeneyeTM 20A kit, were compared with each other and used in the analysis of a special paternity test case. Results The population genetic parameters of 19 STR loci in Shandong Han Population were obtained. The cumulative discrimination power （CDP） and cumulative probability of exclusion （CPE） ranked from high to low were GoldeneyeTM 20A kit, SinoFilerTM kit, PowerPlex 16 kit and IdentifilerTM kit, respectively. As duo case, the result of the real case showed that IdentifilerTM kit had no excluding loci, and none of the SinoFilerTM kit, PowerPlex 16 kit or GoldeneyeTM 20A kit could exclude fatherhood. Conclusion Compared with IdentifilerTM kit, SinoFilerTM kit, and PowerPlex 16 kit, GoldeneyeTM 20A kit shows the higher efficiency than the others, but is not completely satisfied for duo cases.

Key words: forensic genetics, short tandem repeats, polymorphism, genetic, Shandong, Han nationality