Journal of Forensic Medicine

›› 2014, Vol. 30 ›› Issue (4): 282-287.DOI: 10.3969/j.issn.1004-5619.2014.04.013

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Allele-specific PCR and Its Application in Forensic Science

NIE YAN-CHAI1,2, WANG BIN3, ZHAO ZI-QIN1, ZHOU HUAI-GU2   

  1. （1. Department of Forensic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China; 2. Shanghai Key Laboratory of Crime Scene Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 3. Institute of Criminal Science and Technology, Pudong Branch of Shanghai Public Security Bureau, Shanghai 200135, China）
  • Online:2014-08-25 Published:2014-08-28

Abstract:  Allele-specific polymerase chain reaction （AS-PCR） is a technique based on allele-specific primers, which can be used to analyze single nucleotide polymorphism （SNP） effectively including the transition, transversion and insertion/deletion polymorphism and has been exploited in the study of diseases research, molecular diagnosis, and forensic biological evidence. The article systematically reviews the principle, the detection methods, improvement of AS-PCR, and its research updates in the fields of autosome, Y chromosome and mitochondrial SNP, as well as its application in forensic science.

Key words: forensic genetics, polymerase chain reaction, review [publication type], allele specific

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