Journal of Forensic Medicine

›› 2017, Vol. 33 ›› Issue (3): 277-280.DOI: 10.3969/j.issn.1004-5619.2017.03.013

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Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology

YU SHU-XIN1,2, ZENG FA-MING1,3, JIN YAN-ZHANG4, WAN HONG-JING5, ZHAI DIAN3, XING YU-MING1,3, CHENG BAO-WEN1,3   

  1. （1. Kunming Medical University, Kunming 650500, China; 2. Yunnan University of Traditional Chinese Medicine, Kunming 650500, China; 3. Yunnan Provincial Public Security Department, Kunming 650228, China; 4. Longchuan Public Security Bureau, Longchuan 678700, China; 5. Lijiang Public Security Bureau,Lijiang 674100, China）
  • Online:2017-06-25 Published:2017-06-28

Abstract: Objective To detect the genotype of ABO blood group by SNaPshot technology. Methods DNA were extracted from the peripheral blood samples with known blood groups （obtained by serology） of 107 unrelated individuals in Yunnan. Six SNP loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions were detected by SNaPshot Multiplex kit, and relevant genetics parameters were calculated. Results In 107 blood samples, the allele frequencies of types A, B, OA, and OG were 0.355 1, 0.168 2, 0.230 0 and 0.247 6, respectively, while that of types AG and cis AB were not detected. The genotyping results of ABO blood group were consistent with that of serologic testing. Conclusion SNaPshot technology can be adapted for genotyping of ABO blood group.

Key words: forensic genetics, ABO blood group system, genetic testing, SNaPshot technology

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