Abstract: Sex determination is carried out by simultaneouslyamplifying a X-specific and Y-specific DNA frag-ments which are 106hp and 112hp longrespectively in one PCR reaction using one pair OfPrimers which are SPecific to the first intron Of theX-Y homoIogous gene AmeIogenin.PCR productsare Separated on a PAGE electroPhoresis followedby visulization using silver stain.All the bloodStain.muscle,saliva and hair samples gave correctsex determination.Less than or as little as 50pgtemplate DNA and the 16-year-old blood stain allcan achieve satisfactory amplification.The resultsshowed that the method discribed in this paper is arapid,sensitive,reliable and easily manipulated sextest highly adapted to forensic SPecimen especiallythose petrified and degraded.

Key words: sex determination, amelogenin gene, PCR