Abstract: Objective To establish a multiplexing Y-STR system and study haplotype frequencies of 4 Y-specific loci in China Han population. Methods DYS439?DYS390?GATA-A7.2 and DYS393 loci were amplified simultaneously and were analyzed by polyacrylamide gel electrophoresis and silver staining. Results When 558 unrelated male individuals from the Han population in China were tested by the multiplex system, DYS439?DYS390?GATA-A7.2 and DYS393 show 7,7,7,6 alleles, respectively. 180 different haplotypes were detected. The power of discrimination of this system was 0.9853. Conclusion The multiplex amplified system of these 4 Y-specific loci and their database are useful for human origin exploration and forensic practice.

Key words: multiplex amplification, Y-chromosome, short tandem repeats(STR), haplotype