Species Identification of Biological Samples by Amplifying Intron 8 of the TP53 Gene

Abstract

Abstract: Objective To identify the species of biological samples by amplifying intron 8 of the TP53gene. Methods Collected the bloodstains or muscle tissues of 14 kinds of common animals and human.DNA were quantified after extraction.The PCR amplification products were analyzed by PAGE and silverstaining. Results The human and monkey have one amplification band of 460bp while the eel,fish,frog,duck,rabbit,cat,mouse,cavy,pig,ox and sheep could amplify different unspecial bands.No amplificationproducts were found in chicken and dog. Conclusion The method of species identification by amplifyingintron 8 of the TP53 gene is simple and sensitive.

Key words: TP53 tumor suppressor gene, biological samples, species identification

WANG CHUANG;ZHANG LIN;ZHOU BIN;ET AL. (DEPARTMENT OF FORENSIC BIOLOGY;COLLEGE OF PRECLINICAL ANDFORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA). Species Identification of Biological Samples by Amplifying Intron 8 of the TP53 Gene[J]. , 2005, 0(3): 195-196+.

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Species Identification of Biological Samples by Amplifying Intron 8 of the TP53 Gene

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