Archive

28 June 2005, Volume 21 Issue 3 Previous Issue    Next Issue
---
论文


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

论文

Select

ImmunohistochemicalStudyon Expression of iNOSand eNOSduringSkin Incised Wound Healingin Mice ZHAO RUI;GUAN DA- WEI;LU BIN;ET AL.(DEPARTMENT OF FORENSIC PATHOLOGY;CHINA MEDICAL UNIVERSITYSCHOOL OF FORENSIC MEDICINE;SHENYANG 000;CHINA) 2005, 0(3): 161-164+.  Abstract ( 2100 )   ] Objective To investigate the expressions of iNOS and eNOS during skin incised wound healing and theapplicability of time-dependent expressions of iNOS and eNOS to timing of wound. Methods An incised woundwas made in the dorsal skin of mouse . By using S-P method, the expression of iNOS and eNOS were studied in cu-taneous incised wound as well as normal skin in control mice. Results iNOS and eNOS expressed in epidermis,hair follicle, sebaceous gland, and the eNOS were also found in vascular endothelium in control group. Three hoursafter injuryed, the expression of iNOS and eNOS were detected in polymorphonuclear cells (PMNs) in the woundand peripheral region. From 6h to 24h of wound interval, the expression of NOS isoforms were identified in a largenumber of infiltrating PMNs and part of mononuclear cells (MNCs), afterwards the MNCs and fibroblastic cells(FBCs) accounted for the most part of the iNOS and eNOS positive cells. In addition, during wound healing, the ex-pressions of iNOS and eNOS were noticed in different level in vascular epithelial cells, especially neonatal capil-lary vessel in granulation tissue. Conclusion The results suggest that iNOS and eNOS express in polymorphonu-clear cells, macrophages and fibroblasts during healing process of skin incised wound in mice. Furthermore, iNOSand eNOS may play pivotal roles in all the events relating to wound healing, and the time-dependent expressions ofiNOS and eNOS may possibly be used as newmarkers for the wound age determination. Related Articles | Metrics

Select

Expression of β- amyloid Precursor Protein in Diffuse Axonal Injury of Rats ZHU JIN-LONG;ZHU SHAO-HUA;REN LIANG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COL-LEGE;HUAZHONG UNIVERSITY OF SCIENCE & TECHNOLOGY;WUHAN 000;CHINA) 2005, 0(3): 165-168+.  Abstract ( 1738 )   Objective To explore an method for diffuse axonal injury (DAI) diagnosis and injury time esti-mation, the changes of β- APP immunoreactivity and to observe the morphology of axonal in different partsof brain after experimental DAI injury. Methods The animal models of DAI was established according tothe Marmarou′s method. Immunohistochemistry and Gless staining were performed to observe the changesof β- APP expression and the morphology of axon with the time elapsed after the DAI injury. Results Inthe brain injury group, the morphologic changes of axon in brain stem were showed as twisted,broken andswellen at 0.5h , and the myelin sheaths broken could be observed, the retraction ball was found at 12h. Those morphology changes further progressed at 12h, reached to peack up to 1d, then repaired at 3d,and recovered at 10d; Meanwhile the analysis of β- APP immunoreactivity was also showed a time- depen-dent difference as fellows: β- APP expression begin at 3h, increased its immunoreactivity at 12h, reachedto maximize at 1 d, decreased after 3 d, returned to basal level at 10 d. Conclusions The results suggestthat β- APP immunohistochemistry combine with Gless staining be sensitive methods for DAI diagnosis,they could discover the time- dependent changes of the axonal morphology.The changes β- APP are quiteregular and could be used for timing DAI injury. Related Articles | Metrics

Select

Lethal Anaphylactic Shock model Induced by Human Mixed Serum in Guinea Pigs REN GUANG-MU;BAI JI-WEI;GAO CAI- RONG;ET AL.(DEPARTMENT OF FORENSIC MEDICINE;SHANXI MEDICAL UNI-VERSITY;TAIYUAN 0000;CHINA) 2005, 0(3): 169-170.  Abstract ( 2192 )   Objective To establish an anaphylactic shock model induced by human mixed serum in guineapigs. Methods Eighteen guinea pigs were divided into two groups: sensitized and control , The sensitizedgroup were immunized intracutaneously with human mixed serum and then induced by endocardiac injec-tionafter 3 weeks. Results Symptoms of anaphylactic shock appeared in the sensitized group. The level ofserum IgE were increased in the sensitized group significantly . Conclusions An animal model of ana-phylactic shock wer established successfully. It provide a tool for both forensic study and anaphylacticshock therapy. Related Articles | Metrics

Select

Expression of c-Fos in Rats Organs after Electrical Injury WANG YE;LIAO ZHI-GANG;WANG SHI-CHUN;ET AL. (WEST CHINA PRECLINICAL & FORENSIC MEDICINE SCHOOLOF SICHUAN UIVERSITY;CHENGDU 00;CHINA) 2005, 0(3): 171-173+.  Abstract ( 1980 )   Objective To study the expression of c- fos in organs after rats electrified. Methods The brain,lung, heart, liver, spleen, kidney, muscle of electrified limb, and cutis of electrified limb of all experimen-tal rats and those organs of control groups were dissected to detect the expression of c- fos by using immunohis-tochemistry staining, and the staining intensity were assessed by image analysis system. Results The expressionof c- fos was observed in brain, heart, liver, lung, kidney and muscle in electrified directly group, the amount ofexpression reached peak at 2 h after electrified and decreasing at 8 h after electrified, and the expressionshowed faintness in electrified at the immediate after death group. The expressions of c- fos in spleen andcutis is negative in all groups. The expression of c- fos in all detected organs was negative in other ratsthat were electrified after death. Conclusion c- fos can be regard as a target in identifying electrical in-jury, whether it formed at antemortem or postmortem. Related Articles | Metrics

Select

Correlation between PMI and DNA Degradation of Costicartilage and Dental Pulp Cells in HumanBeing LONG REN;WANG WEI-PING;XIONG PING;ET AL. (.DEPARTMENT OF FORENSIC MEDICINE;NANHUA UNIVERSI-TY;HENGYANG 00;CHINA;.PEOPLE′S COURT OF HENGYANG;HENGYANG 00;CHINA) 2005, 0(3): 174-176.  Abstract ( 2137 )   Objective To probe the correlation between the postmortem interval (PMI) and the DNA degradationof costicartilage and dental pulp cells in human being after death ,and to seek a new method for estimating PMI.Methods The image cytometry was used to measure the DNA degradation under different ambient temperatures(30- 35℃,15- 20℃) in 0- 15 days after death. Results The average DNA content of two kinds of tissue wasdegradated with the prolongation of PMI. But there was a plateau period of 0～4 days for dental pulp cells ofhuman being in 15～20℃.There was a high negative correlativity P<0.01 between the average DNA content andPMI. Conclusion PMI could be estimated accurately according to the DNA degradation of costicartilage anddental pulp cells in human being after death. Related Articles | Metrics

Select

Research & Development on computer expert system for forensic bones estimation ZHAO JUN-YI;ZHANG JAN- ZHENG;LIU NIN-GUO(.EAST CHINA UNIVERSITY OF SCIENCE & TECHNOLOGE;SHANGHAI00;CHINA;.INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2005, 0(3): 177-179+.  Abstract ( 1932 )   Objective To build an expert system for forensic bones estimation. Methods By using the ob-ject oriented method, employing statistical data of forensic anthropology, combining the statistical dataframe knowledge representation with productions and also using the fuzzy matching and DS evidence the-ory method. Results Software for forensic estimation of sex, age and height with opened knowledge basewas designed. Conclusion This system is reliable and effective ,and it would be a good assistant of theforensic technician. Related Articles | Metrics

Select

Analysis of Doppler Ultrasonography in Penile Artery ZHU GUANG-YOU;LIU HONG-GUO;CHEN MEI- YING;ET AL. (.INSTITUTE OF FORENSIC SCIENCES;MINISTRY OFJUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA) 2005, 0(3): 180-182.  Abstract ( 1863 )   Objective To study nomal valuels of PI, RI and S/D in healthy male penile dorsum artery(DA) and cavernosal artery (CA). Methods 257 healthy mature men were divided into 5 groups by age.Group 1: <30, n=65; Group 2: 30- 39, n=83; Group 3: 40- 49, n=61; Group 4: 50- 59, n=38; Group 5: ≥60, n=10. Hibateral PI, RI and S/D values of penile dorsum artery and cavernosal artery were examinedby Logidop(r)2 Type Digital Doppler Ultrasonography. Results There were no significant difference for PI,RI and S/D of penile dorsum artery and cavernosal artery not only in personal left and right artery butalso in different age groups. Normal values advised: ①LDA:PI 1.43- 3.43, RI 0.72～0.92, S/D 2.68- 10.56.②RDA PI 1.47- 3.47, RI 0.73- 0.93, S/D 3.27- 10.09. ③LCA:PI 1.49- 3.21, RI 0.74- 0.90, S/D 3.17- 9.55.④RCA:PI 1.93- 3.27, RI 0.72- 0.90, S/D 3.22- 9.42. Conclusion Doppler ultrasonography is a favorablemethod in filtering penile arterial function. Related Articles | Metrics

Select

Application of MSCT and Post-processing Images to Fractures of Nasal Bone in Forensic Identification CHEN XIANG- MIN;LUO SHI- GUANG;WANG ZI- XUAN;ET AL. (.QINGDAO PEOPLE′S HOSPITAL;QINGDAO00;CHINA;.QINGDAO PUBLIC SECURITY BUREAU;QINGDAO 00;CHINA) 2005, 0(3): 183-184+.  Abstract ( 2001 )   Objective To evaluate the application of MSCT and post- processing images to fractures of nasalbone in forensic identification. Methods 134 cases were examined by thin slice scanning with MSCT and all ofthe data were sent to workstation for MPR and SSD. The result of MSCT was compared with that of X- ray. Re-sults There are 55 (41.04%) cases of linear fracture, 46 (34.33%) cases of comminuted fracture, 27 (20.15%)cases of depressed fracture and 6 (4.48%) cases of no fracture in this sample. With X- ray or CR, 48 (35.82%)cases were misdiagnosed or underdiagnosed. 133 (99.25%) cases were confirmed by MSCT. Significance differ-ence was found between X- ray and MSCT (χ2= 45.0816,P<0.001). Conclusion MSCT and post- processing im-ages might be the chief evidence for nasal fractures in forensic identification. Related Articles | Metrics

Select

Anatermic Separated Epiphysis or Avulsion Fracture: Image Observation LI FENG;XU LEI- MING;XIE XING-FU;ET AL. (LONGYOU PEOPLE′PROCURATORCATE;LONGYOU 00;CHINA) 2005, 0(3): 185-187.  Abstract ( 2048 )   Objective To explore an approach to distinguish avulsion fracture of articular portion fromanatomic separated epiphysis. Methods 21 cases with suspicioned avulsion fracture of articular portion oftubular bone were reviewed. The cortical continuity of separate small bone and contiguous bone portionwas investigated. The swell of soft tissue around these small bones was observed simultaneously. Then theconfiguration of the fracture was researched by injury mechanics. Results Continued bone cortex betweenseparate small bone and corresponding bone was seen in 15 cases, so they were considered as a separateepiphysis. Sharp linear fracture between the separate small bone and corresponding bone were seen in 6cases. The swell of soft tissue around the separate small bone was observed in all cases, so they wereconsidered as a avulsion fracture. Conclusion The meticulous investigation of separated small bone at thearticular portion by image observation is of important value for distinguishing avulsion fracture fromanatomic separate epiphysis. Related Articles | Metrics

Select

Genetic Polymorphism Analysis of 6 STR Loci on the X Chromosome in Xi′an Han Population YU BING;QIN QUN- XIA;YAN JIN- CHENG;ET AL. (THE NATIONAL LABORATORY OF THE MINISTRY OF HEALTH FORFORENSIC SCIENCE;COLLEGE OF MEDICINE;XI′AN JIAOTONG UNIVERSITY;XI′AN 00;CHINA) 2005, 0(3): 188-191.  Abstract ( 2020 )   Objective To investigate the alleles and genotypes frequency of 6 short tandem repeat (STR)loci (DXS8378、DXS7132、DXS6789、DXS101、HPRTB and DXS7423) on the X chromosome in Han popu-lation. Methods The six X- chromosome STR loci were analyzed by PCR following polyacylamide gel elec-trophoresis and silver stain. Results Among 120 females from Xi′an Han population, the number of allelesin the 6 loci (DXS8378、DXS7132、DXS6789、DXS101、HPRTB and DXS7423) were 5,6,11,10,8,and 4 re-spectively; the number of genotypes in the 6 loci were 10,17,29,32,22,and 7 respectively; Exact testsdemonstrated genotype frequencies in females had no departure from Hardy- Weinberg equilibrium. Con-clusion The six X- chromosome STR loci are appropriate for individual identification, paternity testing in-volving a female child and for study on related disease. Related Articles | Metrics

Select

Genetic Polymorphism of DXS7423,DXS7424,DXS7132,DXS7133,DXS6804 Loci on X-chromosomein Ewenke Population GU SHAN-ZHI;TONG MU-ER;LI XIAO-ZHONG;ET AL. (THE KEY LABORATORY OF ENVIRONMENT AND GENES RELATEDTO DISEASES OF MINISTRY OF EDUCATION;XI' AN JIAOTONG UNIVERSITY;XI' AN 00;CHINA) 2005, 0(3): 192-194.  Abstract ( 2040 )   Objective To study the genetic polymorphism of five STR loci on X- chromosome in 98Ewenke population of China and establish the related genetic database. Methods The sequence variationof DXS7423,DXS7424,DXS7132,DXS7133,DXS6804 loci on X- chromosome were analyzed by PCR fol-lowing PAG electrophoresis and silver staining. Results The genetic data of the 5 X- chromosome STRs inEwenke ethnic group of China is in accordance with Hard- Weinberg equilibrium. Conclusion Five X- STRloci could be used in forensic identification and population genetic research. Related Articles | Metrics

Select

Species Identification of Biological Samples by Amplifying Intron 8 of the TP53 Gene WANG CHUANG;ZHANG LIN;ZHOU BIN;ET AL. (DEPARTMENT OF FORENSIC BIOLOGY;COLLEGE OF PRECLINICAL ANDFORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2005, 0(3): 195-196+.  Abstract ( 2338 )   Objective To identify the species of biological samples by amplifying intron 8 of the TP53gene. Methods Collected the bloodstains or muscle tissues of 14 kinds of common animals and human.DNA were quantified after extraction.The PCR amplification products were analyzed by PAGE and silverstaining. Results The human and monkey have one amplification band of 460bp while the eel,fish,frog,duck,rabbit,cat,mouse,cavy,pig,ox and sheep could amplify different unspecial bands.No amplificationproducts were found in chicken and dog. Conclusion The method of species identification by amplifyingintron 8 of the TP53 gene is simple and sensitive. Related Articles | Metrics

Select

Diversity of Paternity Index with Different Allele Frequencies LU DE-JIAN;LIU QIU-LING;LU HUI- LING;ET AL. (DEPARTMENT OF FORENSIC SEROLOGY;ZHONGSHAN MEDICAL COL-LEGE OF SUN YAT- SEN UNIVERSITY;GUANGZHOU 00;CHINA) 2005, 0(3): 197-199.  Abstract ( 2261 )   Objective To evaluate the diversity of combined paternity index (CPI) of multiple STR lociwhen different population allele frequencies was used to calculate the paternity index. Methods CPI of 13CODIS (combined DNA index system) loci for 108 trio cases and 108 duo cases selected randomly werecalculated by using five Chinese Han population allele frequencies, respectively. Results The CPI rangefor trio cases and duo cases were 2077.63- 50897711626.46 and 25.12- 2998685141, respectively. Whendifferent population allele frequencies were applied to the same case, the ratio of maximum CPI and min-imum CPI, which was more than 100, for trio cases and duo cases were 20 cases(19.52%) and 13 cases(12.04%) , respectively. Conclusion The variation of CPI value of the CODIS loci was obvious with dif-ferent allele frequencies. To prevent the error causing by uncertain allele frequencies, a conservative CPIvalue should be calculated in paternity testing. Related Articles | Metrics

Select

Study of Rating Scale of Mentally Prisoner′s Competency to Serve a Sentence HUANG FU-YIN;ZHANG QIN-TING;LU CHENG-RONG;ET AL. (INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUS-TICE;P.R.CHINA;SHANGHAI 000;CHINA) 2005, 0(3): 200-202.  Abstract ( 2122 )   Objective To create an instrument to determine the mental prisoners′competency to serve a sen-tence, which is according with the Chinese legal system. Methods Integrating the Chinese criminal jurispru-dence and the authors′forensic psychiatric experience, the research team created an instrument which calledCompetency to serve a sentence Rating scale firstly, then used the instrument retrospectively, in the end the va-lidity and reliability of the instrument were inspected and, through an diagnostic test, the feasibility of the in-strument was evaluated Results Homogeneity reliability of the instrument is 0.8779, the correspondence of theconclusion between the instrument and the expertise is 0.909, except the positive likelihood ratio is 0.0683, theother diagnostic index are better. Conclusion The Competency to serve a sentence Rating Scale is feasible. Related Articles | Metrics

Select

The Method and New Advance In Diagnosing Erectile Dysfunction WANG FEI-XIANG;ZHANG LING-LI(DEPARTMENT OF FORENSIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITYOF SCIENCE AND TECHNOLOGY;WUHAN 000;CHINA) 2005, 0(3): 216-218.  Abstract ( 1856 )   Recently, we have a further understanding on the pathogeny of erectile dysfunction, and with the ap-plication of so many new technologies, such as color Doppler ultrasound, electrical impedance, evoked potential,sensor etc. The diagnosis of ED becomes more and more scientific and convenient. Today we make a review onall kinds of diagnosing methods in order to recognise it more clearly and to provide some more precise andpractical way for forensic evaluation in future. Related Articles | Metrics

Select

Practice Value of Whole Genome Amplification Technology to be Used in Forensic Science and Analysisof its Result DENG JIAN-QIANG;HOU YI-PING (.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI000;CHINA;.INSTITUTE OF FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2005, 0(3): 219-222+.  Abstract ( 1988 )   Genetic analysis from forensic microsamples is a urgent, difficult task in forensic science, because it isfrequently limited by the amount of specimen available in forensic practice, much effort has been carried out toresolve this difficulty. Whole genome amplification (WGA) technology, which was developing quickly in theseyears, has been thought to be a powerful, reliable and efficient strategy in analysis of minute amount DNA onmany fields. In this review, we discuss its application in forensic science. Related Articles | Metrics

Select

The Expression of COX-1 and COX-2 Following Brain Injuries GUO XUE-RONG;ZHOU YI-WU;MA YI-LIE;ET AL. (FACULTY OF FORENSIC MEDICINE;HUAZHONG UNIVERSITY OF SCIENCE& TECHNOLOGY;WUHAN 000;CHINA) 2005, 0(3): 223-225.  Abstract ( 2137 )   Cyclooxygenase (COX) is the obligate, rate- limiting enzyme for the conversion of arachidonic acid intoprostaglandins, which mediate mitogenesis, apoptosis, angiogenesis, blood flow, secondary injury, and inflammation.COX is consi st of 2 subtypes: COX- 1 and COX- 2. In recent years, there are a number of lines of evidencethat COX- 1 and COX- 2 play a important in role brain injuries. Related Articles | Metrics