Abstract: Objective To determinate triptolide and wilforlide A in biological samples by liquid chromatography-tandem mass spectrometry （LC-MS/MS） method and to verify the method. Methods After 0.4 mL blood, urine or 0.4 g hepatic tissues with internal standard were extracted by ethyl acetate, they were separated on a Allure PFP Propyl （100 mm×2.1 mm, 5 ?滋m） with a mobile phase of methanol-20 mmol/L ammonium acetate using gradient elution. For mass spectrometric detection, electrospray ionization （ESI+） in positive mode was elected and the data was collected using multiple-reaction monitoring （MRM）. Results The linearity was good （r>0.995 0） and the limit of detection was 2 ng/mL or 2 ng/g for triptolide and wilforlide A. The recovery was 61.08%-102.98%. The intra-day and inter-day precision was less than 12.58% for each biological sample, and the accuracy was 90.61%-105.80%. Conclusion This method is simple, convenient and good selective, and could be applied to analysis of triptolide and wilforlide A in different biological samples. And the method may provide technical support for forensic medicine identification, clinical diagnosis and treatment of tripterygium wilfordii Hook. f. poisoning.

Key words: forensic toxicology, chromatography, liquid, tandem mass spectrometry, triptolide, wilforlide A, biological samples