Haplotypes of DXS10011 and DXS8377 in Guangdong Han Individuals

Abstract

Abstract: Objective To investigate genetic polymorphism of two X chromosome specific STR: DXS10011 and DXS8377 in male samples from Guangdong Han population. Methods The DNA samples were amplified by PCR and analyzed by polyacrylamide gel electrophoresis followed by silver staining. Results Among 113 samples, 20 alleles were found for DXS1011 and 12 alleles for DXS8377. Also, 72 DXS10011-DXS8377 haplotypes were shown. The most common haplotypes only occurred three times. When only female children were tested in motherless case, the exclusion probability of paternity was 0.9588 for DXS10011-DXS8377 haplotypes. Investigations in 83 family trios with female children and 29 pedigrees with two children suggested a co-dominant X-linked inheritance; mutations were not found. Conclusion Our data indicate that DXS10011 and DXS8377 are highly informative X chromosome markers for complicated kinship analysis.

Key words: short tandem repeat(STR), X chromosome, haplotype, paternity testing

LU DE-JIAN;CHEN SHU-CHUN;WANG XUE-WEN;LIU QIU-LING;LU HUI-LING(DEPARTMENT OF FORENSIC SEROLOGY;ZHONGSHAN-MEDICAL COLLEGEL OF SUN YAT-SEN UNIVERSITY;GUANGZHOU 00;CHINA). Haplotypes of DXS10011 and DXS8377 in Guangdong Han Individuals[J]. , 2006, 0(3): 207-209.

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[2]	Yu-ting WANG, Qiang ZHU, Yu-han HU, Yi-fan WEI, Ting-yun HOU, Ji ZHANG. Calculation of the Paternity Index for the Alleged Father Related to the Child’s Mother [J]. Journal of Forensic Medicine, 2023, 39(3): 271-275.
[3]	Xuan TANG, Dan WEN, Chu-dong WANG, Ru-yi XU, Hong-tao JIA, Jie-nan LI, Bai-yi-la ZHALAGA. Application of Microhaplotypes in Sibling Kinship Testing [J]. Journal of Forensic Medicine, 2023, 39(3): 288-295.
[4]	Chun-yan YUAN, Ruo-cheng XIA, Su-hua ZHANG, Li-qin CHEN, Ya-li WANG, Yi-ling QU, Guang-yuan YANG, Xin-yu DONG, Si-yu CHAI, Cheng-tao LI, Rui-yang TAO. Genetic Polymorphism of 16 X-STR Loci in Xinjiang Uygur Population [J]. Journal of Forensic Medicine, 2022, 38(4): 500-506.
[5]	XIAO Yue, DENG Pan, CHANG Kai-chuang，et al.. Network Analysis of Y-STR in Six Ethnic Populations in Guangxi and Its Forensic Significances [J]. 法医学杂志, 2019, 35(3): 314-318.
[6]	LI Min, HUANG Lei, WANG Xin-jie，et al. Assessment on Application of 24 Y-STR Loci in Forensic Science [J]. , 2018, 34(3): 236-241.
[7]	LUO JI-HUAI, SUN HONG-BING, YANG XIN, ET AL.. Genetic Polymorphism of 24 Y-STR Loci in Dongxiang Ethnic Minority Male Residents in Gansu Province of China [J]. , 2016, 32(3): 219-221.
[8]	LI LI, LIU JUN-HONG, ZHU RU-XIN, ET AL.. Linkage Disequilibrium and Mutation Rate Analysis of Sixteen X-STR Loci [J]. , 2014, 30(6): 437-440.
[9]	NIE YAN-CHAI, ZHANG CHEN, LIU YA-NAN, ET AL.. AS-PCR Assay for 20 mtDNA SNP Typing and Haplotype Frequency [J]. , 2014, 30(2): 96-100.
[10]	SUN KUAN, ZHAO SHU-MIN, ZHANG SU-HUA, ET AL.. Development of an 18 X-InDel Multiplex PCR System [J]. , 2014, 30(2): 101-109.
[11]	ZHANG YIN-MING, LI LI, SUN HONG-YU. Calculation of Likelihood Ratio for X-STR Typing in Kinship Testing [J]. , 2013, 29(3): 180-184.
[12]	MU HAO-FANG, XU DA, LIU BIN, ET AL.. Mutation of Suspected Autosomal STR Loci: 227 Cases Retrospective Analysis [J]. , 2013, 29(3): 196-198.
[13]	YANG PENG, LIU YA-NAN, NIE YAN-CHAI, ET AL. Genetic Polymorphisms of the Dinucleotide STR Locus D6S261 [J]. , 2012, 28(6): 445-447.
[14]	LI LI, ZHAO SHU-MIN, ZHANG SU-HUA, ET AL. Typing and Polymorphism Analysis of 16 STR Loci on X Chromosome [J]. , 2012, 28(1): 36-40,43.
[15]	LI LI, LIU YAN, LIN YUAN, ET AL. Genotyping and Linkage Disequilibrium Analysis of 67 SNP Loci on X Chromosome [J]. , 2011, 27(5): 337-341.

Haplotypes of DXS10011 and DXS8377 in Guangdong Han Individuals

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