Abstract: Objective To obtain population genetic data of loci D11S4951,D11S4957,GATA193H05,D2S2951,and D6S2421 in Han population in Chengdu area and to validate the value of their forensic application. Methods Blood samples were collected in EDTA tubes from unrelated individuals. DNAs were extracted with Chelex-100 and were analyzed by PCR and horizontal PAGE followed by silver staining. Results Alleles 7,10,8,6 and 8 were found in 5 STR loci,respectively. No deviations from Hardy-Weinberg balance were observed. The heterozygosities observed were 0.743,0.772,0.833,0.650 and 0.800,respectively. The chances of exclusion were 0.497,0.549,0.662,0.356 and 0.599,and the discrimination powers were 0.863,0.912,0.947,0.829 and 0.931. Conclusion All of the five loci studied may be useful markers for individual identification and paternity testing.

Key words: short tandem repeats, polymerase chain reaction, electrophoresis, genetics polymorphism