Abstract: Objective To validate the genetic characteristics and distribution of DXS7424-DXS101 on X chromosome in Han population. Methods DXS7424 and DXS101 loci were genotyped by PCR,PAGE and silvers stain methods. Their genetic parameters were analyzed by Arlequin software. Results There were 37 haplotypes detected in 151 Han unrelated males. The frequencies ranged from 0.006 6 to 0.139 1,with a GD value of 0.945 3 and a DP value of 0.938 9. Haplotypes 16-23 were the most common haplotypes in Han population. Conclusion Analysis of combined DXS7424 and DXS101 haplotypes appears to be a powerful means in population genetics and forensic practice for determination of identity and paternity.

Key words: X chromosome, short tandem repeats (STR), haplotype, polymorphism