Abstract: Objective The aim was to investigate the polymorphisms of D6S1043 and D12S391 loci among Han population and evaluate their values in paternity testing. Methods By using fluorescence dye-labeled primers and capillary electrophoresis,the allele frequencies of the two STR loci among 192 unrelated individuals were investigated. Results Twelve alleles were observed in both D6S1043 and D12S391 loci. The ranges of allele frequencies were from 0.002 6 to 0.171 9 and from 0.002 6 to 0.229 2,respectively. The discrimination power of D6S1043 and D12S391 were 0.965 6 and 0.951 0. The Average exclusion probability in paternity testing for duos were 0.573 and 0.510. The Average exclusion probability in paternity testing for trios were 0.731 and 0.679,respectively. The genotypes frequencies met Hardy-Weinberg equilibrium expectation. Conclusion The results show that D6S1043 and D12S391 have high values in forensic paternity testing.

Key words: D6S1043, D12S391, paternity testing, STR