Abstract: Objective To investigate the polymorphism of 11 canine STR loci. Methods A fluorescent multiplex system with 11 STR loci(PEZ1, PEZ2, PEZ3, PEZ5, PEZ6, PEZ8, PEZ12, FH2010, FH2054, FH2132 and FH2611) was constructed independently and performed to amplify 105 samples from dogs. The character of these loci was analyzed with the PCR data. Results The distributions of genotypes and allele frequencies of 11 STR loci were obtained. The total power of discrimination for the 11 loci in canine population was 0.999 999 9 and the cumulative probability of exclusion was 0.933 062 1. The observed heterozygosity and polymorphism information content(PIC) were 0.502 and 0.640, respectively. Conclusion Each of the eleven canine STR loci has a high genetic polymorphism and can be applied for the parentage testing and individual identification. The fluorescent multiplex system is a reliable method in forensic application.

Key words: forensic genetics, canine, genetic polymorphism, STR loci