Abstract: Objective To explore the application of X-chromosomal STR(X-STR) for forensic identification and paternity testing. Methods Six X-STR loci DXS6801, DXS9902, DXS6809, DXS6803, DXS6804 and DXS6799 were amplified in a single PCR reaction. PCR products were analyzed using capillary electrophoresis and 3100 Genetic Analyzer and GeneMapper ID v3.1 Analysis Software. Results The alleles of all six X-STR loci were successfully obtained with unambiguous genotyping, high sensitivity and reproducibility. Conclusion The multiplex PCR of six X-STR loci is useful in forensic identification, particularly for sisters cases.

Key words: forensic genetics, sibling relations, chromosomes, human, X, fluorescent multiplex PCR