›› 2013, Vol. 29 ›› Issue (2): 127-129.DOI: 10.3969/j.issn.1004-5619.2013.02.013

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DNA Extraction from Bones and Teeth Using AutoMate ExpressTM Forensic DNA Extraction System

GAO LIN-LIN1, XU NIAN-LAI2, XIE WEI2, DING SHAO-CHENG1, WANG DONG-JING1, MA LI-QIN3, LI YOU-YING1   

  1. (1. Institute of Criminal Science and Technology, Hangzhou Public Security Bureau, Hangzhou 310004, China; 2. Xiaoshan District Branch of Hangzhou Public Security Bureau, Hangzhou 311200, China; 3. School of Medicine, Zhejiang University, Hangzhou 310058, China)
  • Online:2013-04-25 Published:2013-04-28

Abstract: Objective To explore a new method in order to extract DNA from bones and teeth automatically. Methods Samples of 33 bones and 15 teeth were acquired by freeze-mill method and manual method, respectively. DNA materials were extracted and quantified from the triturated samples by AutoMate ExpressTM forensic DNA extraction system. Results DNA extraction from bones and teeth were completed in 3 hours using the AutoMate ExpressTM forensic DNA extraction system. There was no statistical difference between the two methods in the DNA concentration of bones. Both bones and teeth got the good STR typing by freeze-mill method, and the DNA concentration of teeth was higher than those by manual method. Conclusion AutoMate ExpressTM forensic DNA extraction system is a new method to extract DNA from bones and teeth, which can be applied in forensic practice.

Key words: forensic genetics, bones, teeth, AutoMate ExpressTM forensic DNA extraction system

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