Abstract: Objective To investigate the genetic polymorphisms of 15 X-STR loci in Shandong Han population in order to establish the forensic application database. Methods The multi-PCR primers of these loci were designed by Primer Premier 5.0 software and labeled by 4 fluoresceins （FAM, VIC, NED and TET）. The developed multi-PCR was used to investigate 15 X-STR loci （DXS10011, DXS101, GATA165B12, DXS6795, DXS6800, DXS6801, DXS6803, DXS7132, DXS7133, DXS7423, DXS7424, DXS8377, DXS8378, DXS9898 and HPRTB） selected from the X chromosome of 481 unrelated individuals （295 females and 186 males） in Shandong Han population. Results Among the 15 X-STR loci, GATA165B12, DXS6800, DXS6803, DXS7133 and DXS7423 showed moderate polymorphisms, while the rest 10 X-STR loci showed high polymorphisms （PIC>0.5 and H>0.5）. No shared haplotype was detected among the males in Shandong Han population. Conclusion The developed multi-PCR system with fluorescence detection provides an effective way to establish X-STR loci database of population genetics in Shandong Han population and shows its forensic application.

Key words: forensic genetics, polymorphism, genetic, X-STR, Shandong, Han nationality