The Effects of Heroin on Intracellular Free Ca~(2+) of Rat Myocardium

Abstract

Abstract: Objective To observe the effects of heroin on intracellular free Ca2+ in rat myocardium. Methods The effects of heroin on intracellular free Ca2+ were observed in cultured neonatal rat myocardium by measuring intracellular free Ca2+ concentration using calcium fluorescent probe Flou-3/AM and laser scanning confocal microscope. Results Different doses and concentrations of heroin appeared to have different effects on intracellular free Ca2+ concentrations, with a dosage dependent short linear increase in the fluorescence intensity (i.e., Ca2+concentration) leading to [Ca2+]i peak. Conclusion Heroin could affect concentrations of [Ca2+]i in myocardium and its dosage related effect needs further investigation.

Key words: heroin, myocardial cells, free, Ca2+

LIU XIAO-SHAN;WU JING-DONG;HAO ZI-RUI;LIU SHUI-PING;LI ZHAO-HUI;LI HAI-XIA;CHEN YU-CHUAN (. DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE OF SUN YAT-SEN UNIVERSITY;GUANGZHOU 000;CHINA;.SHIQI BRANCH OF PUBLIC SECURITY BUREAU OF ZHONG. The Effects of Heroin on Intracellular Free Ca~(2+) of Rat Myocardium[J]. , 2007, 0(6): 424-427.

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The Effects of Heroin on Intracellular Free Ca~(2+) of Rat Myocardium

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