Abstract: Objective To study the changes of HSP 70 mRNA and c-fos mRNA expression and to find a method to differentiate antemortem from postmortem electrocution.Methods Fifteen New Zealand rabbits were randomly divided into three groups,the antemortem electrocution group,the postmortem electrocution group,and the control group.Each group consists of five rabbits.The levels of HSP 70 mRNA and c-fos mRNA in skeletal muscle and cardiac muscle were examined with quantitative fluorescent RT-PCR.Results The levels of HSP 70 mRNA and c-fos mRNA in the antemortem electrocution group increased significantly(P<0.05),compared with that of the postmortem electrocution group.Conclusion The changes of HSP 70 mRNA and c-fos mRNA expression in skeletal muscle and cardiac muscle can be used as an indicator to distinguish antemortem from postmortem electrocution.

Key words: forensic pathology, electric injuries, flurphotometry, HSP 70 heat-shock proteins, genes, fos, reverse transcriptase polymerase chain reaction