Archive

28 August 2008, Volume 24 Issue 4 Previous Issue    Next Issue
---
论文


For Selected:

Download Citations EndNote Ris BibTeX

Toggle Thumbnails

论文

Select

The Time-dependent Changes of Phospho-JNK Expression during the Skin Incised Wound Healing in Mice XIONG CHANG-YAN;GUAN DA-WEI;YANG MEI;ZHAO RUI;ZHENG JI-LONG;WANG LING;YU TIAN-SHUI;CHENG ZI-HUI;HU GENG-YI;ZHU BAO-LI(SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA) 2008, 0(4): 241-244.  Abstract ( 2284 )   Objective To investigate the changes of phospho-JNK(p-JNK) during the incised wound healing of the skin in mice and to explore the rule of the time-dependent change of p-JNK in wound age determination.Methods The changes of p-JNK expression in incised skin wound were detected by immunohistochemistry and Western blot.Results There was a minimal baseline staining of p-JNK in control mouse skin.Changes of p-JNK expression were mainly detectable in neutrophils in the wound specimens from 3 hours to 12 hours after injury.Afterwards,the p-JNK positive cells were mostly mononuclear cells and fibroblasts between post-injury day 1 and day 5,whereas the p-JNK positive cells were mostly fibroblasts between post-injury day 7 and day 14.Morphometrically,the ratio of the p-JNK positive cells to the total increased gradually in the wound specimens from 3 hours to day 1,and maximized at day 1 with a slight decrease from post-injury day 3 to day 5.The ratio showed a second peak in the specimens of day 7,and then decreased gradually from post-injury day 10 to day 14.The changes of p-JNK expression were observed throughout the wound healing stages by Western blot as well,with a peak expression occurring between 12 hour and day 3 after injury.Conclusion p-JNK may play a pivotal role in inducing apoptosis of neutrophils,mononuclear cells,and fibroblasts during skin wound healing and meanwhile,p-JNK may be a potentially useful marker for wound age determination. Related Articles | Metrics

Select

The Expressions of HSP 70 mRNA and c-fos mRNA in the Skeletal Muscle and Cardiac Muscle of Rabbits by Electrocuted WANG YE;LIU MIN;CHENG WEI-BO;HE GUI-QIONG;LI FAN;LIAO ZHI-GANG(.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.WEST CHINA SCHOOL OF PUBLIC HYGIENICS;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.HEN 2008, 0(4): 245-247+.  Abstract ( 1866 )   Objective To study the changes of HSP 70 mRNA and c-fos mRNA expression and to find a method to differentiate antemortem from postmortem electrocution.Methods Fifteen New Zealand rabbits were randomly divided into three groups,the antemortem electrocution group,the postmortem electrocution group,and the control group.Each group consists of five rabbits.The levels of HSP 70 mRNA and c-fos mRNA in skeletal muscle and cardiac muscle were examined with quantitative fluorescent RT-PCR.Results The levels of HSP 70 mRNA and c-fos mRNA in the antemortem electrocution group increased significantly(P<0.05),compared with that of the postmortem electrocution group.Conclusion The changes of HSP 70 mRNA and c-fos mRNA expression in skeletal muscle and cardiac muscle can be used as an indicator to distinguish antemortem from postmortem electrocution. Related Articles | Metrics

Select

Comparison of Thresholds Acquired with ASSR and PTA in Normal-hearing Subjects YANG XIAO-PING;FAN LI-HUA;ZHOU XIAO-RONG;ZHU GUANG-YOU(SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE;INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2008, 0(4): 248-251.  Abstract ( 2019 )   Objective To provide new objective technology in evaluating hearing loss for forensic expert by comparing the thresholds acquired with auditory steady-state responses(ASSR) and pure tone audiometry(PTA)in our laboratory.Methods Twenty-seven subjects with normal hearing(54 ears,18-28years)were tested with PTA and ASSR at 500,1 000,2 000 and 4 000 Hz.Paired t-test analysis was performed on threshold of ASSR and PTA at difference frequency(500,1 000,2 000 and 4 000 Hz).Results At each frequency(500,1 000,2 000 and 4 000 Hz),the thresholds acquired with ASSR were higher than those acquired with PTA(P<0.05).The mean difference of each frequency were(22.04±5.79) dB(500 Hz),(11.02±5.44) dB(1 000Hz),(12.59±5.89) dB(2 000 Hz) and(17.78±7.25) dB(4 000 Hz).The mean difference between the system automatically adjusted threshold of ASSR and PTA were(-3.96±5.79) dB at 500 Hz,(0.02±5.44) dB at 1 000 Hz,(-0.41±5.89) dB at 2 000 Hz,and(-1.25±7.25) dB at 4 000 Hz.Conclusion At each frequency(500,1 000,2 000 and 4 000 Hz),the thresholds acquired with ASSR were higher than those acquired with PTA.The mean difference is 16.9 dB.As an objective and mature method,ASSR could be used in evaluation of hearing-loss degree for forensic expert.But adjusted threshold value should be established when ASSR is used to estimate hearing threshold in each laboratory. Related Articles | Metrics

Select

Assessment of Skeletal Age in Chinese Male Adolescents WANG PENG;ZHU GUANG-YOU;WANG YA-HUI;FAN LI-HUA;ZHANG GUO-ZHEN;YING CHONG-LIANG;CHENG YI-BIN;LU XIAO(.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL SCHOOL OF SOOCHOW UNIVERSITY;SUZHOU;CHINA;.SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE;INSTI 2008, 0(4): 252-255+.  Abstract ( 1852 )   Objective To develop medicolegal expertise standard to assess skeletal age of Chinese male adolescents by joint X-ray.Methods Radiographs of male adolescent aged 11 to 20 years were obtained in Southern,Central,and Eastern China.The bone development process were graded according to morphology characteristics in radiograph based on the appearance of secondary ossification centers and the arrest of epiphysises.All statistical analysis was carried out with SPSS and SAS.Results Most indexes were correlated to age.Optimization multiple regression equation was created to estimate age of adolescent.Discriminatory equations were established to distinguish the age of 14,16 or 18 years.Conclusion More anatomical site evaluation and more index application can improve the accuracy rate.The methods can be used in age determination by skeleton in Chinese male adolescents. Related Articles | Metrics

Select

An Examination of the Self-reported Scale of Brief Psychopathological Symptoms to Detect Malingering in Forensic Psychiatric Subjects GAO BEI-LING;HUANG ZHI-BIAO;WU DONG-LING;DING SHU-MING;LIANG WEI-DE;LI XUE-WU(FORENSIC PSYCHIATRIC INSTITUTE OF SHENZHEN KANGNING HOSPITAL;SHENZHEN 00;CHINA) 2008, 0(4): 256-258.  Abstract ( 1976 )   Objective To examine the self-reported scale of brief psychopathological symptoms(SBPS) to detect malingering in forensic psychiatric cases.Methods Two hundred and six cases with different types of psychiatric problems were tested by SBPS.All cases were separately evaluated by two experts.Results About 34.5% cases(71/206) were classified as malingering by the cut-off 13 scores of SBPS.Compared with expert′s evaluation,SBPS showed a false negative rate of 19.8% and a false positive rate of 1.7%,respectively,with a total accuracy rate of 90.8%.Cases involved in compensations including working injury and traffic accidence showed the highest rate of malingering(51%).Conclusion SBPS is useful for detecting malingering psychopathological symptoms. Related Articles | Metrics

Select

Multiple Amplification of 16SrRNA Gene and Cytb Gene in Mitochodrial DNA for Species Identification YE YI;WU JIN;LUO HAI-BO;WANG ZHUO;LI YING-BI(DEPARTMENT OF FORENSIC BIOLOGY;WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA) 2008, 0(4): 259-261.  Abstract ( 2180 )   Objective To establish a fluorescent multiple amplification system of 16SrRNA and Cytb genes located in mitochondrial DNA for species identification.Methods A pair of primers of 16SrRNA gene and Cytb gene of the mitochondrial DNA was designed with the software Primer 5.0 to construct a multiple amplification system.The amplified products from human and five species of animals,including cattle,pig,dog,chicken and grass carp were analyzed by 310 Genetic Analyzer.Results The amplified products of these samples showed two peaks.The common one was 358 bp and the specific one different in unique species was between 231 bp and 256 bp.Conclusion The multiplex amplification system can exactly distinguish the species of human from five common animals. Related Articles | Metrics

Select

DNA Polymorphism Detection of Papaver Somniferum L Using Fluorescent Amplified Fragment Length Polymorphism LU FAN;CHENG BAO-WEN;LI HONG;HONG JUN-JIE;SUN HAI-YAN;ZHAO WEN-SONG;YANG HONG-MEI;LI JIN;ZHAO YU-RONG(.INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY;YUNNAN PROVINCE POLICE BUREAU;KUNMING 00;CHINA;.KUNMING MINGJI SCIENCE AND TECHNOLOGY CO.;LT 2008, 0(4): 262-264+.  Abstract ( 2016 )   Objective To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.Methods Genomic DNA was isolated using the AxyPrep DNA Kit,double-digested by two restrictional endonucleases(Eco RI and Mse I) and ligated to oligonucleotide adapters.After Pre-amplification and selective amplification,the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.Results More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.Conclusion The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum. Related Articles | Metrics

Select

The Relation of Blood Alcohol Concentration and Neurobehavioral Functions after Drinking ZHUO XIAN-YI;BU JUN;XIANG PING;SHEN BAO-HUA(SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE;INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2008, 0(4): 265-267.  Abstract ( 1931 )   Objective To research the relation between blood alcohol concentration(BAC) and neurobehavioral function after drinking.Methods The neurobehavioral ability index(NAI) of 233 volunteers were measured with computer-administered neurobehavioral evaluation system-Chinese3(NES-C3).Results The NAI of simple visual reaction time and mental arithmetic declined when BAC was more than 0.157 mg/mL,the NAI of benton visual retention,length discrimination and digit cancel declined significantly when BAC was more than 0.204 mg/mL.Conclusion The neurobehavioral function declined significantly when BAC increased and recovered gradually when BAC declined due to the elimination of alcohol in blood. Related Articles | Metrics

Select

Simultaneous Analyses of Cocaine and its Metabolite Benzoylecgonine in Urine by LC-MS/MS SUN QI-RAN;XIANG PING;YAN HUI;SHEN MIN(.DEPARTMENT OF FORENSIC SCIENCE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA;.SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE;INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHAN 2008, 0(4): 268-272.  Abstract ( 1862 )   Objective To establish a liquid chromatography-tandem mass spectrometry(LC-MS/MS) method for the simultaneous analysis of cocaine(COC) and its metabolite benzoylecgonine(BZE) in urine samples.Methods A single dose of COC(10 mg/kg) was injected intraperitoneally into guinea pigs and urine samples were collected for 7 days.The urine samples were extracted by auto solid phase extraction(SPE),separated by the Allure PFP propyl column with a mobile phase consisting of methanol and 20 mmol/L ammonium acetate buffer 0.1% formic acid(80:20,V/V),and then analyzed by LC-MS/MS.Multiple reaction monitoring(MRM) mode was used to analyze COC(m/z 304.2→182.3,m/z 304.2→150.1) and BZE(m/z 290.2→168.3,m/z 290.2→105.0).Results COC and BZE showed a fairly good linearity over the range of 2.0～100 ng/mL(r=0.999 5).The detection limit was 0.5 ng/mL.The recovery rate was greater than 90% and the deviation of intra-and inter-day precision was less than 6%.BZE was the major target detected in urine samples,and its detection window was longer than COC.Conclusion This newly developed method shows high sensitivity and selectivity,and is suitable for the simultaneous analysis of cocaine and benzoylecgonine in urine samples. Related Articles | Metrics

Select

A Forensic Pathological Study of Eighty-two Cases of Adrenal Hemorrhage CHENG WEN-TING;YI XU-FU;LU JIE-LIANG;HE QIN-PIAO;ZHONG JIAN-SHENG;LIAO ZHI-GANG(.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.RENHUA PUBLIC SECURITY BUREAU;SHAOGUAN 00;CHINA;.XINHUI BRANCH OF 2008, 0(4): 273-274+.  Abstract ( 1739 )   Objective To analyze the relationship between adrenal hemorrhage and the cause of death,age and gender.Methods Eighty-two cases of adrenal hemorrhage were statistically analyzed.Results Adrenal hemorrhage occurred mostly in cases of sudden death,infection,trauma and asphyxia.Male had more chance than female to have adrenal hemorrhage.Adrenal hemorrhage caused by sudden death,trauma and poisoning was more frequently seen in young adults,whereas adrenal hemorrhage in children as well as in fetus and newborns was often caused by infection as well as sudden death and asphyxia respectively.Adrenal hemorrhage caused by sudden death and asphyxia was mainly located in medulla,while the infection usually induced hemorrhage in cortex.Adrenal hemorrhage caused by trauma showed an equal opportunity in either the cortex or medulla.Conclusion Our data indicate that adrenal hemorrhage might provide some clues in searching for the cause of death. Related Articles | Metrics

Select

Advances in Research of Diagnosis of Drowning in Forensic Medicine WANG JIA-WEN;YU XIAO-JUN;WANG XIAO-YAN(.DEPARTMENT OF FORENSIC MEDICINE;MEDICAL COLLEGE;SHANTOU UNIVERSITY;SHANTOU 0;CHINA;.MEDICAL SCHOOL OF FOSHAN UNIVERSITY;FOSHAN 00;CHINA) 2008, 0(4): 276-279.  Abstract ( 2073 )   The current macroscopical and microscopical signs in the fresh drowning victim are non-specific.Moreover,putrefaction will vanish these autopsy findings quite rapidly.The study of additional detecting methods to diagnose drowning has become one of the hottest topics in forensic medicine.This article reviewed several methods commonly used in the determination of drowning in forensic practice.The advantages and disadvantages of various methods were also discussed. Related Articles | Metrics

Select

Electrophysiological Examination of Peripheral Nerve Injury and its Significance in Forensic Medicine ZHANG XIN-YUAN;LIU JI-HUI;CUI YONG;TANG PENG(DEPARTMENT OF CLINICAL FORENSIC MEDICINE;SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA) 2008, 0(4): 280-283.  Abstract ( 1857 )   Assessment of peripheral nerve injury is a common task in forensic practice.As an objective assistance of inspection,electrophysiological examination is significant in the evaluation of peripheral nerve function after injury.There are currently several methods of electrophysiological examination,including I-T curve,EMG,NCV and SEP.Because electrophysiological examination can assess the injury and regeneration of peripheral nerve injury at different times,it is valuable to estimate prognosis of peripheral nerve injury in forensic medicine.Besides the electrophysiological examination,evaluation of the peripheral nerve injury needs to combine other clinical manifestations to provide an accurate appraisal. Related Articles | Metrics

Select

The Relationship between SNP of Cholecystokinin Gene and Certain Mental Status and its Forensic Significance YANG JUN;WANG BAO-JIE;DING MEI;PANG HAO;SUN XUE-FEI;LI ZHONG-JIE(SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA) 2008, 0(4): 284-287.  Abstract ( 1898 )   Cholecystokinin(CCK) is a brain-gut peptide with broad biological activities.It is one of the main regulating hormones in the digestive system,and it plays an important physiological role in the central and peripheral nervous system as a neurotransmitter or a neuromodulator.Recently,many reports have demonstrated that there were a number of SNPs on CCK gene,of which -45C/T and -196G/A showed certain correlation with a variety of psychiatric states such as schizophrenia,depressive disorder,suicidal behavior,Parkinson′s disease,etc.These SNPs may be used in paternity testing and personal identification.In addition,it may also become one of the auxiliary indicators in some special cases of forensic pathology. Related Articles | Metrics

Select

Detection of Malingering Post-traumatic Stress Disorder HUANG LE-PING;XIE BIN(SHANGHAI MENTAL HEALTH CENTER;SHANGHAI JIAOTONG UNIVERSITY;SHANGHAI 0000;CHINA) 2008, 0(4): 288-292.  Abstract ( 1840 )   Assessment of post-traumatic stress disorder is a frequently encountered problem in forensic practices.Detection of malingering has become a focus in these assessments.While it is a difficult task due to kinds of traumatic events,complex clinical symptoms,subjective medical information,and presence of diverse psychotic disorders.The clinical traits,detecting methods and testing instruments of PTSD malingering were reviewed so as to help practical management and assessment. Related Articles | Metrics

Select

Tramadol Intoxication and Its Toxicological Effect WANG HUA-XIN;WANG LING;GUAN DA-WEI;WANG MING-BIAO(.DEPARTMENT OF FORENSIC PATHOLOGY;SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA;.DEPARTMENT OF PATHOLOGY AND MEDICAL SCIENCE;COLLEGE OF BASIC MEDICAL SCIENCES;DALIAN ME 2008, 0(4): 293-296.  Abstract ( 1915 )   Tramadol is a centrally acting analgesic with a dual mode of action.Its analgesic efficacy is attributed to its partial affinity for the μ-opiate receptor and its inhibition of norepinephrine and serotonin reuptake.Acting in a synergistic manner and being more efficacious,tramadol is used worldwide for the treatment of moderate to severe acute or chronic pain.Abuse and dependence of tramadol as well as tramadol-related deaths have been increasingly reported,either ingested alone or taken in combination with other potentially interacting drugs.The possible toxic effect of tramadol was reviewed from aspects of its analgesic mechanisms,adverse effect,dependence,and abuse. Related Articles | Metrics

Select

Discussion on Medical Malpractice in Traumatic Injury YAN ZHI-WEI(HENAN ADMINISTRATIVE INSTITUTE OF POLITICS AND LAW;ZHENGZHOU 000;CHINA) 2008, 0(4): 297-300.  Abstract ( 1877 )   By reviewing five medical tangle cases after traumatic injury,the characters of these cases and the cause of medical malpractice had been discussed.After the analysis of the responsibility to the death caused by traumatic injury and medical malpractice,the assessment principle of cooperative competition of causes and degree of participation had been introduced.Meanwhile,it is believed that if comprehensive postmortem examination and pathologic examination were carried out to verify the death cause at first,they would play more important role in the appraisal of medical tangle cases after traumatic injury. Related Articles | Metrics