Abstract: Objective To detect DNA polymorphism of Papaver somniferum L using fluorescent Amplified Fragment Length Polymorphism.Methods Genomic DNA was isolated using the AxyPrep DNA Kit,double-digested by two restrictional endonucleases(Eco RI and Mse I) and ligated to oligonucleotide adapters.After Pre-amplification and selective amplification,the DNA fragments were separated by capillary electrophoresis using the CEQ8000 DNA Fragment Analyzer.Results More than 20 fragments of highly polymorphic products were obtained by 8 pairs of primer from 64 selective amplifying primer pairs.Conclusion The fluorescent AFLP technique can be used to detect the DNA polymorphism of Papaver somniferum.

Key words: forensic medicine, botany, papaver somniferum L, amplified fragment length polymorphism