Journal of Forensic Medicine

›› 2010, Vol. 26 ›› Issue (5): 340-342.DOI: 10.3969/j.issn.1004-5619.2010.05.006

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Determination of Bloodstain Formation Time by RNA Analysis

XU YAN1, JIANG WEI2, PING YUAN1, BI GANG1, CHEN LIAN-KANG1, ZHOU HUAI-GU1   

  1. （1. Shanghai Key Laboratory of Crime Science Evidence, Key Laboratory of Forensic Evidence and Science Technology, Ministry of Public Security, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. Hongkou Branch of Shanghai Public Security Bureau, Shanghai 200434, China）
  • Online:2010-10-25 Published:2010-10-28

Cited

6

Abstract: Objective To investigate the expression of 18S rRNA and β-actin mRNA in bloodstain between 8 and 15 days after death and extrapolate the time of bloodstain formation. Methods RNA in dried bloodstain at different times was extracted, then quantified for 18S rRNA and β-actin mRNA by real-time RT-PCR.  The bloodstain formation time was deduced based on the changes of the ratio of 18S rRNA to β-actin mRNA at different time points. Results The ratio of 18S rRNA to β-actin mRNA increased gradually with time, indicating that rRNA and mRNA degraded  in different rate with time. Conclusion The ratio of 18S rRNA to β-actin mRNA could be used for estimating the time of bloodstain formation in some period.

Key words: forensic genetics, reverse transcriptase polymerase chain reaction（RT-PCR）, time of bloodstain

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