Abstract: Objective To investigate the changes of creatine kinase-MB （CK-MB） and heat shock protein 60 （HSP 60） in rats without electric marks after electric injury, to identify the relationship of the CK-MB, HSP 60 and the time of electric injuries, and to evaluate the damage to cells after electric injury. Methods The animal model of electric injury without electric marks was established by alternating current （voltage 110 V）. Automatic biochemistry analyzer was used to detect the serum CK-MB and immunohistochemical staining technology was used to analyze the tissues of myocardium and left lobe of liver. Results The amount of serum CK-MB was increased when the rats were injuried, and reached the peak at 30 min. Then the amount of CK-MB began to decrease and showed a slight downward trend in 3-5 h after electric injury, and leveled off at 6 h. Immunohistochemistry staining also showed the changes of HSP 60 of rats’ myocardial cells and hepatic cells regularly after electric injury. Conclusion The regular changes of serum CK-MB and tissular HSP 60 in rats can be used to diagnosis electric injury and assess the injury of internal organs after the electric injury without electric marks.

Key words: forensic pathology, electric injuries, creatine kinase, MB form, heat-shock proteins, rats