Abstract: Objective To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit. Methods One thousand nine hundred and forty-eight samples （including samples spotted on FTA cards, filter papers and case samples） were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler^TM kit, Identifiler kit and PowerPlex 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit. Results 97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with Sinofiler^TM kit, Identifiler kit and PowerPlex 16 kit at the same STR loci. Conclusion Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results and be applied to forensic DNA genotyping.

Key words: forensic genetics, STR, Expressmarker 22 STR loci direct PCR amplification kit