Abstract: Objective To evaluate the forensic application value of 30 insertion/deletion （InDel） loci included in Investigator® DIPplex Kit in Han and She nationalities of Eastern China. Methods A total of 565 unrelated individuals in Han nationality and 119 ones in She nationality of Eastern China were investigated using Investigator?誖DIPplex Kit. Allele frequencies, population genetics parameters of the 30 InDel loci were statistically calculated. Results In Han nationality, the mean Ho was 0.413 3, the mean DP was 0.551 1, the mean PIC was 0.320 0. And in She nationality, the mean Ho was 0.389 6, the mean DP was 0.543 3, the mean PIC was 0.310 0. No deviation from Hardy-Weinberg equilibrium was observed in Han and She nationalities （P>0.05）. Conclusion The 30 loci in Investigator?誖DIPplex Kit show good genetic diversity in Han and She nationalities, and could be used as a supplemental tool for some special paternity cases.

Key words: forensic genetics, polymorphism, genetic, InDel, Investigator®DIPplex kit, Han nationality;She nationality