Journal of Forensic Medicine

›› 2016, Vol. 32 ›› Issue (5): 356-362.DOI: 10.3969/j.issn.1004-5619.2016.05.010

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Genetic Polymorphisms of 21 STR Loci in Hunan Province-based Han Population

ZOU YING1, GUO JUAN-JUAN1, LI QING-PENG2, ZUO DAO-HONG2, LIU JIN-SHAN3, GUO YA-DONG1, YAN JIE1, ZHA LAGABAIYILA1, CAI JI-FENG1, LAN LING-MEI1   

  1. （1. Department of Forensic Science, School of Basic Medical Sciences, Central South University, Changsha 410013, China; 2. Xiangya School of Medicine, Central South University, Changsha 410013, China; 3. Department of Internal Medicine, Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao 028000, China）
  • Online:2016-10-25 Published:2016-10-28
  • Contact: LAN Ling-mei, M.S., experimentalist, major in forensic genetics; E-mail:lanlingmei0731@163.com
  • About author:ZOU Ying, M.S., experimentalist, major in forensic genetics; E-mail: zouhawk@163.com
  • Supported by:

    the Natural Science Foundation for the Youth （No. 81302621） and the Fundamental Research Funds for the Central Universities of Central South University （2015zzts276）

Abstract: Objective To investigate the genetic polymorphisms of 21 short tandem repeat （STR） loci （D3S1358, D13S317, D7S820, D16S539, Penta E, D2S441, TPOX, TH01, D2S1338, CSF1PO, Penta D, D10S1248, D19S433, vWA, D21S11, D18S51, D6S1043, D8S1179, D5S818, D12S391 and FGA）. Methods A total of 560 blood samples were collected from unrelated healthy individuals of Han population in Hunan Province. Chelex-100 extraction method was applied to the extraction of genomic DNA, and an AGCU EX22 Kit and 9700 STR amplification was used in amplification reactions. The products were separated and analyzed on 310 Genetic Analyzer. Results A total of 248 alleles were observed, the allelic frequencies ranging from 0.001 to 0.518. Observation of genotype distributions for each locus showed no deviations from Hardy-Weinberg equilibrium except Penta E （P=0.023）. The combined power of discrimination, combined power of exclusion, and combined matching probability of the 21 STR loci were approximately 0.999 999 999 999 999 999 999 999 8, 0.999 999 998, and 1.36×10-25, respectively. Conclusion The 21 STR loci show high polymorphisms in the Han population, which can provide valuable data and a theoretical basis for forensic individual identification and paternity testing.

Key words: forensic genetics, polymorphism, genetic, short tandem repeat sequences, Hunan, Han nationality

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