Abstract: Objective To explore the application value of InnoTyper?誖21 kit in forensic practice. Methods Samples of hair shafts and saliva were collected from 8 unrelated individuals. Template DNA was extracted by AutoMate ExpressTM forensic DNA automatic extraction system. DNA was amplified by InnoTyper?誖21 kit and AmpF?詛STRTM IdentifilerTM Plus kit, respectively, and then the results were compared. Results After the amplification by InnoTyper?誖21 kit, complete specific genotyping could be detected from the saliva samples, and the peak value of genotyping profiles of hair shafts without sheath cells was 57-1 219 RFU. Allelic gene deletion could be found sometimes. When amplified by AmpF?詛STRTM IdentifilerTM Plus kit, complete specific genotyping could be detected from the saliva samples, and the specific fragment was not detected in hair shafts without sheath cells. Conclusion The InnoTyper?誖21 kit has certain application value in the cases of hair shafts without sheath cells.

Key words: forensic genetics, insertion/deletion, hair shaft, personal identification, InnoTyper?誖21 kit