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28 August 2007, Volume 23 Issue 4 Previous Issue    Next Issue
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The Expression of Ubiquitin in the Healing Process of Incised Wounds of Rat's Skin and its Significance to Forensic Medicine ZENG YI;DENG SHI-XIONG(DEPARTMENT OF FORENSIC MEDICINE;CHONGQING UNIVERSITY OF MEDICAL SCIENCES;CHONGQING 000;CHINA) 2007, 0(4): 241-243+.  Abstract ( 2128 )   Objective To evaluate the changes of ubiquitin expression in incised wounds of the rat skin.Methods The testing rat groups were subjected to incised skin wounds,with normal rat skin used as control.The expression level of ubiquitin was assessed using immunohistochemistry and imaging analysis technique on skin samples taken at 1,3,6,12 h,and on day 1,3,6,10,and 14 d after injury.Results The expression level of ubiquitin was low in the skin of normal control group.Increased level of ubiquitin expression could be observed 1 h after injury.The expression level of ubiquitin reached its peak on day 6 and started to decline on day 10,and then returned to its normal level on day 14 d after injury.Conclusion Ubiquitin may serve as a potentially useful marker for forensic determination of the skin wound age. Related Articles | Metrics

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Corpus of Biochemical Changes after Death by Potassium Intoxication in Rabbits ZHU XIN-JU;LI XI-YUAN;LI KAI;CHEN LI-PING;KE YONG;WANG ZHEN-YUAN(.FORENSIC MEDICINE DEPARTMENT;MEDICAL SCHOOL OF XI'AN JIAOTONG UNIVERSITY;XI'AN 00;CHINA;.ZHENJIANG PUBLIC SECURITY BUREAU;ZHENJIANG 000;CHINA;.YIJUN PUBLIC SECURITY BUREAU 2007, 0(4): 244-246+.  Abstract ( 1747 )   Objective To explore the objective evidence of the corpus biochemical changes in rabbits for postmortem diagnosis of potassium intoxication.Methods Rabbits were sacrificed by Infusion of 0.3% KCl at full speed push or 1% KCl at 100 drip/min,respectively,with normal rabbits used as control.Cardiac blood and urine samples were collected before and after potassium infusion to examine the concentrations of various electrolytes(K+,Na+,Ca2+,Mg2+,Cl-,and HCO3-) and to observe the antemortem and postmortem biochemical changes.Results The mean lethal infusion time in the 0.3%KCl group was longer than that in the 1% KCl group(P=0.006).The serum concentration of K+ increased while the serum concentrations of Na+,Ca2+,Cl-,and HCO3-decreased after the infusion.There were no statistically significant differences in the whole blood concentration of K+ as well as the serum concentration of Mg2+ between the two groups(P=0.062).There were statistically significant differences in the concentrations of whole blood K+,as well as serum Na+,Mg2+,and Cl-,but not in the serum K+,Ca2+,and HCO3-.There were no statistically significant differences seen in the urine volumes and the concentrations of all the urine electrolytes between the groups.Conclusion Examination of the concentrations of K+ both in the whole blood and serum,as well as Mg2+ in the serum may be helpful for postmortem diagnosis of potassium intoxication. Related Articles | Metrics

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The Expression of Coxsackie B Virus Adenovirus Receptor(CAR) in Viral Myocarditis and Dilated Cardiomyopathy Patients LI HONG-YAN;LI YONG-HONG;ZHU TUAN-JIE(.FORENSIC MEDICINE DEPARTMENT OF WANNAN MEDICAL COLLEGE;WUHU 00;CHINA;.PUBLIC SECURITY BUREAU OF JIANGSU PROVINCE;NAJING 00;CHINA) 2007, 0(4): 247-249+.  Abstract ( 2143 )   Objective To explore etiology and pathogenesis of viral myocarditis(VMC) and dilated cardiomyopathy(DCM).Methods The expression of Coxsackie B virus and adenovirus receptors(CAR) were detected with modified immunohistochemical(IHC) technique in myocardium of left ventricle,right ventricle,interventricular septum,and septal papillary muscle from 28 patients with viral myocarditis,31 patients with dilated cardiomyopathy and 17 control patients(including normal,hypertension heart disease,myocardial infarction and coronary atherosclerotic heart disease).Results The brown staining on the cell membrane of myocardium represents positive result.100%(28 of 28) of VMC patients(IHC surface integral: 4.397 5±0.036 5) and 84%(26 of 31) of DCM patients(4.206 4±0.052 6) had prevalent CAR expression compared to 0%(0 of 19) control patients(0.073 1±0.036 2).There were statistically significant differences between VMC/DCM and control patients(P<0.05).Conclusion The prevalence of CAR expression was significantly higher in VMC and DCM patients(100% and 84% VS 0% in control).In contrast,there was no difference found between VMC and DCM patients.These results suggest that both VMC and DCM involve viral etiology and could share a similar pathogenesis. Related Articles | Metrics

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Simulation Study on the Bicyclist Involved Traffic Accident and Analysis of the Injury ZOU DONG-HUA;LIU NING-GUO;CHEN JIAN-GUO;ZHANG JIAN-HUA;JIN XIAN-LONG;ZHANG XIAO-YUN;CHEN YI-JIU (.INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN 2007, 0(4): 250-253+.  Abstract ( 1755 )   Objective This study aimed at computer simulation of reconstruction of vehicle-bicyclist traffic accidents and investigation of the injury characteristics.Methods To use the model established by high performance computer and PC-Crash software to study the bicyclists and vehicles and to reconstruct the accident.Results The model of computer simulation response perfectly reconstructed phases of the traffic collision and explained the mechanisms of the injuries of the bicyclists,which correlated with details found in forensic investigation.Conclusion Computer simulation is feasible for forensic analysis on the injury mechanism of bicyclists in traffic related accidents. Related Articles | Metrics

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Comparison of Several Visual Evoked Potential Tests in Measuring Normal Adults′ Real Visual Acuity XIA WEN-TAO;ZHU GUANG-YOU(.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 0000;CHINA) 2007, 0(4): 254-257.  Abstract ( 2168 )   Objective To study and compare several measures of pattern visual evoked potential in measuring normal adults′ real visual acuity.Methods Forty normal eyes were selected to test the pattern reversal visual evoked potential,black-white scanning pattern visual evoked potential,red-black scanning pattern visual evoked potential,respectively.Their experiment visual acuity was compared with their real visual acuity.Results The black-white scanning pattern visual evoked potential and the red-black sweep pattern visual evoked potential were more accurate,more convenient,and much faster in measuring real visual acuity than the pattern reversal visual evoked potential.Conclusion Pattern reversal visual evoked potential,black-white scanning pattern visual evoked potential,and red-black scanning pattern visual evoked potential all can be used in forensic medical examination of eye injuries. Related Articles | Metrics

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Accuracy of Age Estimation from Orthopantomograph Using Demirjian's Method TAO JIANG;WANG YI;LIU RUI-JUE;XU XIAO;LI XIAO-PENG(.SHANGHAI NINTH PEOPLE′S HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE;SHANGHAI 000;CHINA;.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;C 2007, 0(4): 258-260.  Abstract ( 1892 )   Objective Developing teeth are used to assess maturity and estimate age in a number of disciplines.The aim of this study was to determine the accuracy of Demirjian method(Panoramic radiographs were examined and seven mandibular teeth staged according to Demirjian′s dental maturity scale) in the forensic clinical medicine.Methods Tooth formation was assessed with orthopantomographs in healthy children in dental teaching hospital.There were total 828 children,with 279 boys and 549 girls,aged from 11 to 19 years.The difference between dental and real age was compared and measured,using t-test.Results The Demirjian method overestimated age in the aged 11-16 years group and had limitations in aged group over 17 years.Conclusion The 95% confidence interval of the mean was least for mean of all developing teeth using Demirjian method(age 11-16 years). Related Articles | Metrics

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Forensic Medical Assessment in Post-trauma Retinal Detachment LIU RUI-JUE;XIA WEN-TAO;FAN LI-HUA(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2007, 0(4): 261-264+.  Abstract ( 2127 )   Objective To analyze risk factors associated with post-traumatic retinal detachment and to identify the cause-effect relationship between retinal detachment and blunt ocular trauma in forensic medical assessment.Methods 112 cases of forensic medical examination on post-traumatic retinal detachment were retrospectively analyzed.Results A variety of retinal abnormalities were found in these cases,including tearing of the ora serrata retinae(4.28%),macular perforation(12.50%),small(<90°) non-macular retinal perforation(56.25%),larger(>90°) retinal perforation(5.00%),and tractive retinal detachment without perforation(11.6%).Proliferative vitreoretinopathy(PVR) was found in 45 eyes examined.In most cases,retinal detachment occurred between 1 week to 2 months after blunt ocular trauma(61.60%),with 83.93% accompanied with severe myopia(>-3.00D) and 52.67% accompanied with vitreous floaters.Of all cases,41.07% were directly and 52.68% were indirectly resulted from blunt ocular trauma,and the rest(6.25%) showed no association with blunt ocular trauma.Conclusion Many risk factors may result in retinal detachment including blunt ocular trauma and other causes.Accurate assessment of the relationship between blunt ocular trauma and retinal detachment is an important part of forensic examination. Related Articles | Metrics

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Anatomy Study of MGA in Chinese and its Effect on Legal Expertise SHEN YI-WEN;ZHENG RU;WANG TAO;LUO PENG-BO;HE MENG;WU RONG-QI;JIA JIAN-ZHANG;XUE AI-MIN;ZHAO ZI-QIN(.DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA;.HUA SHAN HOSPITAL OF FUDAN UNIVERSITY;SHANGHAI 2007, 0(4): 265-268.  Abstract ( 2159 )   Objective This study aimed to clarify the morphology of the Martin-Gruber anastomosis(MGA) in Chinese.Methods One hundred and five Chinese upper limbs(36 males and 20 femalese) were dissected to find the connections between medial nerve and ulnar nerve.The MGA was classified as previously described by Lee.Results MGA was found in 24 cases(22.9%),in 11 of the 36 male and 5 of the 20 female.There was no obvious difference in the frequency of MGA in both upper limbs.Most MGA ulnar position was located at the medial and distal segment of the forearm.Conclusion MGA anatomy could play important role in forensic diagnosis of ulnar nerve injury in Chinese population. Related Articles | Metrics

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Epidemiological Research and Analysis on the Impaired Person in Road Traffic Accident in Chengdu Area HUANG SI-XING;LI SHENG-YAN;ZHANG XIAN-GUO;KONG BIN;ZHU YA-LI;LIU KUAN-LIN(.TRAFFIC ADMINISTRATION DEPARTMENT OF CHENGDU MUNICIPAL PUBLIC SECURITY BUREAU;CHENGDU 00;CHINA;.TRAFFIC POLICE SUB-BRIGADE OF NANCHONG MUNICIPAL PUBLIC SECURITY BUREA 2007, 0(4): 269-273.  Abstract ( 1705 )   Objective To analyze epidemiological characteristics of road traffic accidents that resulted in injuries in Chengdu area,to find out the cause and to provide scientific a base for accident prevention.Methods Appraisal data of the injured in road traffic accident from January 2003 to October 2006 were re-examined.Detailed statistics were made which include gender,age,transportation mode,person types,the accident date and day,and location.Results Six thousand three hundred and sixty four cases involving road traffic accident were analyzed.Among the injured,male to female ratio was 1.84:1,and the age group of 18-50 were the largest proportion(66.34%).Bicyclists,motorcyclists and pedestrians made up 80.90% of the injured persons.Different age groups,different genders and those who were injured in different regions varied greatly in terms of transport modes they employed.In terms of injured parts,lower limb injury took up the highest proportion(39.49%),with cranium & cerebrum ranking second(22.77%).Conclusion Different approaches and measures shall be taken in regard to different age groups,different regions,different times and different transport modes,and corresponding policies shall be adopted. Related Articles | Metrics

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Evaluation of Liquid Based Cytology Test(LCT) in Avoiding Medical Tangles LIU BAO-QIN;YAN ZHAO-WEN;YAO HONG-XIA;CAI JI-FENG(.MEDICAL COLLEGE OF SHANGHAI JIAOTONG UNIVERSITY;SHANGHAI 000;CHINA;.MEDICAL COLLEGE OF TONGJI UNIVERSITY;SHANGHAI 0000;CHINA;.XIANGYA SCHOOL OF MEDICINE;CENTRAL SOUTH UNIVERSITY;CHANGSHA 00 2007, 0(4): 274-276.  Abstract ( 1791 )   Objective To evaluate of liquid based cytology test(LCT) in avoiding medical tangles.Methods One thousand five hundred five thirty one cases,which were collected from out-patients of precancerous lesions of uterine cervix,were randomly divided into three groups based on different smear preparation:LCT method was used in two groups(one with ThinPrep R kit and one with ArtoBrain R kit),conventional Papauicolaou smear(PS) was used in one group.All cases of abnormal cervical smears were identified by cytologic test underwent colposcopic examination and colopscopically multiple biopsy.Results of test were analyzed by software SPSS 11.0.Results Significant diference were found between LCT method and PS method compared by index of satifacation,sensitivity,specificity,accuracy,false negative rate and erroneous diagnosis rate(P<0.05,but no difference were found between two LCT groups(ThinPrep R kit and ArtoBrain R kit).Conclusion LCT method can improve diagnostic level of precancerous lesions of uterine cervix either tested by ThinPrep R kit or ArtoBrain R kit,so have the powerfull value to avoid medical tangles. Related Articles | Metrics

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Influencing Factors of Psychopath's Civil Litigation SUN HUI-YAN;HUANG FU-YIN;CAI WEI-XIONG;GUAN WEI;ZHANG QIN-TING;WU JIA-SHENG;WANG JIAN-JUN (.MEDICAL SCHOOL OF SOOCHOW UNIVERSITY;SUZHOU 00;CHINA;.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2007, 0(4): 277-279+.  Abstract ( 2092 )   Objective To explore the influencing factors of schizophrenic patient's capability in civil litigation,and to establish the base of quantitative study about execution of civil litigation.Methods To study questionnaires completed from patients with and without civil litigation capabilities and to determine the influencing factors from medical and forensic aspects.Results One hundred patients were admitted to the study and were divided into two groups based on capability in civil litigation.There were significant differences in psychiatric and legal aspects between the groups.Conclusion Capability of schizophrenic patients in perusing civil litigation had been impaired or even complete lost. Related Articles | Metrics

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Evaluation of Allelic Alterations in 13 CODIS STR Loci in Tumor Tissues from Human Digestive System FANG JIAN-XIN;LI CHENG-TAO;XIAO LI (. INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA;. SHANGHAI HUANG DONG HOSPITAL;SHANGHAI 000;CHINA) 2007, 0(4): 280-282.  Abstract ( 2224 )   Objective To study genetic alterations in 13 CODIS STR loci in various tumor tissue samples from human digestive system. Methods Malignant tumor tissues and blood samples taken from 55 different unrelated individuals were collected. DNA samples were extracted using Chelex100 extraction kit, amplified using Profiler and Cofiler PCR amplification kit and analyzed using API 310 analyzer. Results Aberrant cell divisions were detected in all of the 55 tumor tissue samples, with STR alternations detected in two samples including allelic alteration, partial and complete loss or unbalance of heterozygosity. Moreover, the alternations might occur simultaneously at more than one loci. Conclusion Caution must be taken in STR analysis of tumor tissue samples since the exclusion loci in forensic identification or paternity testing may be resulted from mutations in the tumor tissue. Related Articles | Metrics

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Molecular Genetic Analysis of Diego Blood Group Dia and Dib in Chinese Han Population YANG BAO-CHENG;SU YU-QING;YU QIONG;WEI TIAN-LI;LI DA-CHENG;LIANG YAN-LIAN(SHENZHEN BLOOD CENTER;SHENZHEN INSTITUTE OF TRANSFUSION MEDICINE;SHENZHEN 0;CHINA) 2007, 0(4): 283-285.  Abstract ( 2079 )   Objective To study the molecular genetic background of Diego blood group in Chinese Han population.Methods A total of 2 990 blood samples from unrelated blood donors were phenotyped for Dia and Dib by serological method.Twenty randomly selected samples of Di(a-b+) type and all of the samples of rare Di(a+b-) phenotype by screening were genotyped by PCR-SSP and direct DNA Sequencing.Results Of the 2 990 samples identified by serological method,2 821 were Di(a-b+),167 were Di(a+b+) and 2 were Di(a+b-).All of the 20 randomly-selected samples with Di(a-b+) phenotype were DI2DI2 homozygote by PCR-SSP genotyping,with nucleotide C at nt position 2 561 in exon 19 by direct sequencing of the DI gene.The 2 samples of rare Di(a+b-) phenotype were both the DI1DI1 homozygote,with nucleotide T at nt position 2561 in exon 19.Conclusion Our results indicate that the expression of Dia and Dib antigens in Chinese Han population most likely result from a single nucleotide T to C substitution at nucleotide position 2561 in exon 19 of the DI gene,which subsequently leads to an amino acid 854 change from Pro to Leu. Related Articles | Metrics

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Isolating Sperm Cells and Preparing DNA from Mixture Sample LI XIN;HU LAN;FENG XUE-FEI;LIU XIAO(.CHINESE PEOPLES PUBLIC SECURITY UNIVERSITY;BEIJING 000;CHINA;.ZIBO PUBLIC SECURITY BUREAU;ZIBO 000;CHINA;.INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF PUBLIC SECURITY;BEIJING 000;CHINA;.FORENSIC MEDICAL 2007, 0(4): 286-289.  Abstract ( 1754 )   Objective To establish a method of preparing and analyzing DNA of sperm cells from sample mixtures.Methods Sperm cells were captured by micromanipulation method,DNA template was prepared from these sperm cells,and then amplified by multiple displacement amplification(MDA).Results High-yield of highly integrated DNA was produced by MDA from very small amount of DNA template of 20 sperm cells.The DNA could be amplified at least 30 000-fold,and the length of most the DNA segments were greater than 15kb,providing sufficient amount of short tandem repeat(STR) products for DNA genotyping.Conclusion The micromanipulation method is efficient to isolate sperm cells from sample mixtures and multiple displacement amplification can provide sufficient amount of DNA products for STR genotyping.The method seems to be feasible in detecting DNA from rape cases. Related Articles | Metrics

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Mutations of Short Tandem Repeat Loci in Identifiler~(TM) System ZHAO ZHEN-MIN;LIU YAN;LIN YUAN (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA) 2007, 0(4): 290-291+.  Abstract ( 2107 )   Objective To explore and analyze the mutations of 15 Short Tandem Repeat (STR) loci using IdentifilerTM system in paternity identification. Methods 2 712 cases of paternity testing were carried out using IdentifilerTM PCR Amplification Kit. Results Of the 2362 paternity testing cases, mutations of single locus were observed in 51 cases. The mutation loci included D8S1179, D21S11, D7S820, CSF1PO, D3S1358, D13S317, D16S539, D2S1338, D19S433, vWA, D18S51, D5S818 and FGA, with the D21S11 locus having a highest mutation rate (0.369%). Thirty-six of the STR mutations were from paternal source, 7 from maternal source, and the rest (9) were undeterminable. The mutation rates at D21S11 were highest (0.369%). Conclusion Mutations of STR loci are relatively common in human genome. Therefore, re-testing of additional relatively stable STR loci with lower mutation rates is necessary when one or two loci exclusions are encountered in paternity testing. Related Articles | Metrics

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A Study in Extraction Method of Buprenorphine in Urine LIU DONG-XIAN;HE JIANG-NAN(DEPARTMENT OF CRIMINAL TECHNOLOGY;HUNAN PUBLIC SECURITY COLLEGE;CHANGSHA 0;CHINA) 2007, 0(4): 292-294.  Abstract ( 2029 )   Objective The extraction method for separating buprenorphine from urine was described.Methods Buprenorphine was extracted with chloroform at pH7 or with SPE(extracted by organic support 401 at pH10.8,then eluted with chloroform),finally determined by GC-NPD.Results The extracted yields of the analyte in specimens were 86.6% by solution-phase extraction and 83.0% by solid-phase extraction(SPE) respectively.Conclusion These two methods are simple and accurate for separating buprenorphine from urine. Related Articles | Metrics

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Forensic Analysis on Injuries Involving Main Branches of Brachial Plexus in 66 Cases LU HONG;HU BIN-WEI;HUANG LI-NA;LI QI(SHANGHAI CRIMINAL SCIENCE AND TECHNOLOGY INSTITUTE;SHANGHAI 000;CHINA) 2007, 0(4): 295-298.  Abstract ( 2036 )   Objective To study injuries involving brachial plexus and its branches.Methods 66 cases collected from 2003 to 2005 in our department were analyzed statistically.Results 94% of injuries involved young adult males;94% were blunt force injuries;34% involved both nerve and bone mainly involving ulnar nerve and ulnar bone(50%);40% of the injured nerves received electromyogram and 15 nerves were diagnosed with injuries clinically.Conclusion Forensic determination on severity of brachial plexus injuries mainly depends on movement recovery of limb.The best time for forensic appraisal is 20 d post operation+L(length of nerve severed distally)/R(growth rate)+90 d. Related Articles | Metrics

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The Retrospective Study of Sudden Cardiac Death in 118 Cases HE KE;XU JIAN-SHE;WANG ZHEN-YUAN (. KEY LABORATORY OF ENVIRONMENT AND GENE RELATED TO DISEASES OF MINISTRY OF EDUCATION;DEPARTMENT OF FORENSIC SCIENCE;SCHOOL OF MEDICINE OF XI’AN JIAOTONG UNIVERSITY;XI’AN 00;CHINA;.PEOPLE’S PROCURATORATE 2007, 0(4): 299-301.  Abstract ( 1988 )   The sudden cardiac death (SCD) is the primary cause of death in adults. 118 cases of sudden cardiac death from 1998 to 2005 in Shanxi area were studied. The results showed that there as 5.9 times of males as females. There were 55.1% coronary artery diseases, 17.8% myocarditis, 9.3% cardiomyopathy, 2.5% each of congenital heart diseases, valvular heart diseases, and rupture of dissecting aneurysm of aorta, and 4.55 of other diseases. The data indicate that SCD happened in males more often than in females and coronary artery disease was the most common disease. Myocardititis was more common in adolescents and cardiomyopathy was more prevalent in young adults resulting in SCD. The cardiac disease without abnormal structure changes might also be a serious threat to human health. Related Articles | Metrics

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DNA Genetyping of the Trace Bloodstains on the Adsorbent Object CHEN RONG-HUA;SONG QING;XU QIN-WEN;DONG YAN(.SHANGHAI INSTITUTE OF CRIMINAL SCIENCE AND TECHNOLOGY;SHANGHAI 000;CHINA;.JIADING BRANCH OF SHANGHAI PUBLIC SECURITY BUREAU;SHANGHAI 000;CHINA) 2007, 0(4): 302-303.  Abstract ( 1937 )   Objective To study the extraction and analysis of DNA from trace bloodstains on the adsorbent object.Methods DNA was extracted by Chelex-100,QIAamp Mini Kit,and modified QIAamp Mini Kit,respectively.The extracted DNA was amplified and short tandem repeat(STR) loci were genotyped by multiplex PCR procedures,and then analyzed by ABI 3100 Genetic Analyzer.Results The STR loci could be better genotyped with modified QIAamp Mini Kit,compared to poorer genotyping results obtained with Chelex-100 extraction method and QIAamp Mini Kit.Conclusion Our data indicate that DNA extracted from trace bloodstains on adsorbent object with modified QIAamp Mini Kit serves a better DNA template for amplification and genotyping. Related Articles | Metrics

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Application of MiniFiler Kit in Forensic DNA Testing of Low Copy Number Template TANG JIAN-PING;WU DAN;ZHANG CHEN;ZHOU HUAI-GU (INSTITUTE OF FORENSIC SCIENCE;SHANGHAI MUNICIPAL PUBLIC SECURITY BUREAU;SHANGHAI 000;CHINA) 2007, 0(4): 304-306.  Abstract ( 2032 )   Objective To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit(MiniFiler) in forensic practice.Methods Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer(Applied Biosystems,Foster City,USA).Results DNA samples as low as 10 pg could be amplified by MiniFiler kit,and the optimal DNA quantity was 40 pg or above.Conclusion MiniFiler kit can be used for analysis of low copy number STR. Related Articles | Metrics

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Analysis on The Abused Drugs in Saliva and Correlation of Saliva Drugs Concentrations with Blood Concentrations LI PENG-WANG;WANG YU-JIN;LIU JUN-FANG(SCHOOL OF FORENSIC MEDICINE;SHANXI MEDICAL UNIVERSITY;TAIYUAN 0000;CHINA) 2007, 0(4): 309-311+.  Abstract ( 2073 )   Saliva is an easily collected body fluid and has simple composition.Some drugs concentrations in saliva can reflect their blood level.This paper analyzes the mechanisms of drug transfer from blood into saliva and the influencing factors,reviews the methods of sample collection,preparation,analysis of the abused drugs in saliva,and the relationship between abused drugs content in saliva and in blood.We believe that saliva is a valuable sample in clinic and forensic medicine.It is important in forensic science field to estimate abused drugs concentrations in blood by via their saliva concentrations. Related Articles | Metrics

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A Reviewing for Abusing of Ketamine YANG JU;BIAN SHI-ZHONG(DEPARTMENT OF FORENSIC MEDICINE;MEDICAL SCHOOL OF SOOCHOW UNIVERSITY;SUZHOU;CHINA) 2007, 0(4): 312-315.  Abstract ( 2087 )   Ketamine is a noncompetitive NMDA receptor antagonist and comes into being a new problem of drug abuse.It can cause a certain extent of hallucination,which makes ketamine be abused in the casinos.The paper reviews the pharmacological and toxicology characteristic of Ketamine,the possible physiological mechanism and the methods for detecting Ketamine abuse. Related Articles | Metrics