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    28 February 1991, Volume 7 Issue 1 Previous Issue    Next Issue

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    Humen Hairs Sex is Identified in Analysis Amplified DNA Sequences
    WU MEIYIUN
    1991, 0(1): 1-3+14. 
    Abstract ( 1901 )  
    We have used PCR to detect DNA sequences from root region of single, freshly-plucked hairs and single, hairs plucked half a year ago. Our results demonstrated that DNA isolated from freshly-plucked hairs and hairs of half a year old was undegraded as compared with DNA digested with Hind III. The amount of DNA isolated from sin -gle, freshly-plucked hairs was arround 200 ng, that from single, plucked hairs of half a year old was arround 133 ng. It was found that both 154-bp and 130-bp fragment of DNA were obtained from male's hairs while 130-bp fragments was obtained from female's hairs. The 154-bp fragment is the expected product with the Y-specific sequence primers 1.1 and 1.2. The 130-bp fragment is the expected product with the A1u sequence primers 9.1 and 9.2. 1/3 amount of DNA isolated from single, freshly-plucked hairs was enough for ampli-fication with one pair of primers. Sex determination with 2/3 DNA can be achieved.
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    Studies on apisination by observation of bee sting under Scanning Electron microscope (SEM)
    HUANG RUITING DEPARTMENT OF FORENSIC MEDECINE;HIGH COUNT OF JUSTICE;FUJIAN PROVINCE;FUZHOU
    1991, 0(1): 6-7+2+. 
    Abstract ( 1934 )  
    For the forensic pathologist, anaphylatic fatality (bee sting) is a diagnosis derived more by exclusion (e.g. toxin, trauma and natural disease) than by inclusion. As in all medicolega problems, anaphylatic fatalities are nonspecific and are easy to confuse the following. ①other anaphylactogens(e.g.drug、plant、food、vaccine、parasite、pollen or antiserum); ②laryngeal edema (may be caused by allargic or anaphlactic reactions、infection、 tumors or asresult of an irritant gas); ②unexplained deaths. This paper reports the results of the morphology of bee sting in skin under Scanning Electron microscopy (SEM). our studies show that this method makes progress in the identification of beesting and provides an objective indentify target. In order to improve the quality of examine continually, this paper also put forward a proposal using knowledge of entomology, zoology and botang to solve the medicolega problems.
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    The Studies on Plant-hemagglutinin
    DEPT. OF FORENSIC BIOLOGY SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES CHAI RUIBO;GUO YUNRONG
    1991, 0(1): 8-11. 
    Abstract ( 1910 )  
    We collected 329 kinds of plant-seeds. It was found that there were 60 kinds of plant-seeds whose extract solutions could agglutinate human erythrocytes to various extents (the most hemagglutinating titer was 2048x)using agglutination test, neutralization test and absorption test. The extract solutions of 23 kinds of plant-seeds could not be inhibited by saliva-stains using neuyralization test. The extract solutions of some plant-seeds (13/23) could be inhibited by extract solutions of seminal stains. Some (5/23) could be inhibited by extract solutions of bloodstains. The results of absorption test showed that the most of extract solutions of plant-seeds (19/23) could be absorbed by bloodstains. It remains to be further studied whether or not these neutralization tests, inhibition tests and absorption tests are specific and if these planthemagghitinins can be used practically.
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    A Study of Toxins From Castor Beans——Section Ⅰ A Simplified Method of Isolating Toxins from Castor Beans
    WU YONGHONG;ZHANG PENG;YAN PINHUA INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;PRC
    1991, 0(1): 12-14+3. 
    Abstract ( 2087 )  
    We simplified the method of isolating toxic ingredients (ricin, lectin and some other complicated proteins) from castor beans. Some of their properties were also tested and compared with each other.
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    A Study of Toxins From Castor Beans——Section Ⅱ A Study of Toxication by Toxins from Castor Beans
    ZHANG PENG;WU YONGHONG;YANG JIANPING INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;PRC
    1991, 0(1): 15-16+3. 
    Abstract ( 1917 )  
    In this paper, we poisoned four groups of mice with the two main toxic ingredients of castor beans. The pathological changes of the poisoned mice were observed in comparison with a control group. Some new findings, especially the degeneration of myoc were made.
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    The Distribution of Haptolobin Subtype Gene Frequencies in Xian District and Their Application to Legel Medicine
    CONG BIN HEBEI MEDICAL COLLEGE LIU MINGJUN;HU BINWEI;XIAN MEDICAL UNIVERSITY
    1991, 0(1): 17-20. 
    Abstract ( 2105 )  
    We examined the Hp subtypes both in the human sera and in the dried bloodstains using a method for Hp purifacation, the hydrolysis of Hp performed using the β-merca- ptoethanol-ureaiodacetamid mixture and the isoelectric focusing electrophoresis on slab polyacrylamide gel. Hp phenotyping of 404 serum samples from unrelated chinese blood donors in Xian were carried out. The distribution of the Hp subtypes are: Hp~(2SS)0.25%, Hp~2F~S49.5%, Hp~(1S)6.68%, Hp~(2fS-2SS)4.21%, Hp~(2SS)-~(1S)1.49%, Hp~(2fs)-~(1S)35.89%, Hp~(2fS)-~(1S)0.495%, Hp~2-~S-~(2V)0.743%, Hp~(1S)-~(1f)0.25% and Hp~(2V)-~(1S)0.495%. The gene frequencies respectively are: Hp~(1f)0.0037, Hp~(1S)0.2574, Hp~(2fS)0.0309, Hp~(2fS)0.7017 and Hp~(2V)0.0063. The observed and expected values provided a good fit to the Hardy-Weinberg equilibrium(p>0.05). The probab ility of excluding paternity means 0.2601, and discrimination probability means 0.62. The 24 samples of bloodstains were kept in room tempreture for seven days, 4℃ for seven days and -30℃ for thirty days. In addition to three samples, the others could besubtyped agreeing with the subtype of Hp in their serum.
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    The Phenotype Distribution of the Red Cell Glyoxalase I in Talyuan Area and Phenotyping of Glyoxalase I in Bloodstansi
    LIANG JINGQING;MAO SHUZANG DEPARTMENT OF FORENSIC MEDICINE;SHANXI MEDICAL COLLEGE
    1991, 0(1): 21-22+3. 
    Abstract ( 1886 )  
    The 290 blood samples from Taiyuan area were detected by mixed starch/agarose gel electrophoresis. The phenotypes were 8 GIOI_(1-1), 63 GLOI_(2-1), 219 GLOI_(2-2). The gene frequencies were: GLOI~1 0.1362; GLOI~2 0.8638. The 50 bloodstain samples on the cloth kept at room temperature for 3 weeks could be phenotypod correctly. The staining method was improved. The background of gel plank is clear. The improved method is (simple) rapid and more practical.
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    Legal Expertise of Paranoid Psychosis
    WU JIA-SHENG
    1991, 0(1): 37-39. 
    Abstract ( 1942 )  
    Four murder cases of paranoid psychosis are reported in the present paper. As this kind of mental disorder is relatively less common and the symptoms of it are not so remarkable as to be easily recognized, the characteristics, especially clelusion of jealousy have been discussed. And the law problems, such as evaluation of criminal responsibility, etc, have been put under discussion too. It is summarized that in the work in identification the symptom of dlelussian of jealousy has to be firstly noticed in this case, the patient has no criminal responsibility at all.
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