法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (3): 324-327.DOI: 10.12116/j.issn.1004-5619.2019.03.012

• 技术与应用 • 上一篇    下一篇

不同免疫学检验方法对混合精斑的检测及应用

董敏安1，2， 李美丽1， 蔡铭升1   

  1. 1. 广州医科大学基础医学院，广东 广州 510000； 2. 湛江市公安局，广东 湛江 524000
  • 发布日期:2019-06-25 出版日期:2019-06-28
  • 通讯作者: 李美丽，女，博士，副教授，硕士研究生导师，主要从事生物化学与分子生物学以及免疫学和病原生物学研究；E-mail：meili_2004163.c@163.com
  • 作者简介:董敏安（1986—），男，主要从事法医物证学研究；E-mail：donma033x@vip.qq.com

Analysis of Detection of Mixed Semen Stains with Different Immunological Test Methods and Case Applications

DONG Min-an1,2, LI Mei-li1, CAI Ming-sheng1   

  1. 1. School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou 510000, China; 2. Zhanjiang Public Security Bureau, Zhanjiang 524000, Guangdong Province, China
  • Online:2019-06-25 Published:2019-06-28

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摘要： 目的 利用免疫学检验方法对混合精斑进行分离确证，寻找更有效的混合精斑检测方法。 方法 将3名志愿者的精液混合后，分别用前列腺特异性抗原（prostate-specific antigen，PSA）胶体金免疫试纸条法、免疫磁珠法以及激光捕获显微切割技术对混合精斑进行处理和检测，对分型结果进行统计，并与单一精斑结果进行比较。 结果 经PSA胶体金免疫试纸条法测试，各样品在测试区和控制区内均出现一条紫红色条带；经免疫磁珠法处理后，检出更完整有效的短串联重复（short tandem repeat，STR）分型；应用激光捕获显微切割技术对混合精斑进行处理并经低体积扩增后，将结果汇总、叠加获得完整的单一分型，与单一精斑分型相匹配，分型成功率为84.00%。把上述方法应用于实际案件中，获得了单一的嫌疑人Y-STR分型，为案件的快速破获提供了证据基础。 结论 联合应用PSA胶体金免疫试纸条法、免疫磁珠法以及激光捕获显微切割技术可快速对混合精斑进行分离检验。

关键词: 法医遗传学, 短串联重复, Y染色体, 混合精斑, 免疫胶体金, 免疫磁珠, 激光捕获显微切割

Abstract: Objective To perform the separation and confirmation of mixed semen stains with immunological test method, and find a more effective method for the detection of mixed semen stains. Methods The semens of three volunteers were mixed. The mixed semen stains were processed and tested with prostate-specific antigen （PSA） colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection, respectively. Statistics of the results of STR were gathered and compared with those of a single semen stain. Results After PSA colloidal gold immunoassay strip method testing, the samples showed a purplish red line in the test area and the control area. The results obtained with the immunomagnetic beads method showed a more complete and effective short tandem repeat （STR） sequence. The mixed semen stains were processed with laser capture microdissection and low volume amplified. The results were summarized and superimposed to obtain a complete single typing, which matched the single semen stain typing, with a typing success rate of 84.00%. Single suspect Y-STR typing was obtained with the application of the method above in actual cases, which provided evidence basis for rapid solving of the case. Conclusion The combination of PSA colloidal gold immunoassay strip method, immunomagnetic beads method and laser capture microdissection can be used to separate and confirm the mixed semen stains.

Key words: forensic genetics, short tandem repeat, Y chromosome, mixed seminal stain, immunocolloidal gold, immunomagnetic bead, laser capture microdissection