法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (4): 393-395，401.DOI: 10.12116/j.issn.1004-5619.2019.04.002

• 论著 • 上一篇    下一篇

大鼠脑组织电导率及相关生化指标与死亡时间的关系

孙凯， 郑哲， 李永林， 赵琳琳， 莫耀南   

  1. 河南科技大学法医学院，河南 洛阳 471003
  • 发布日期:2019-08-25 出版日期:2019-08-28
  • 通讯作者: 莫耀南，男，教授，硕士研究生导师，主要从事法医病理学和法医临床学研究；E-mail：forensic@haust.edu.cn
  • 作者简介:孙凯（1991—），男，硕士研究生，主要从事法医病理学研究；E-mail：kaisun1992@126.com 通信作者：
  • 基金资助:
    河南省研究与院士工作站资助项目（13610002）

Relationship between Electrical Conductivity and Related Biochemical Indicators of Rat Cerebrum Tissues and Postmortem Interval#br#

SUN Kai, ZHENG Zhe, LI Yong-lin, ZHAO Lin-lin, MO Yao-nan   

  1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, China
  • Online:2019-08-25 Published:2019-08-28

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摘要： 目的 探究大鼠脑组织浸渍液电导率（electrical conductivity，EC）及其相关生化指标与死亡时间（postmortem interval，PMI）的关系，探讨应用EC推断PMI的机制。 方法 将40只健康SD大鼠处死后保存于25 ℃左右的环境中，分别于死后0、1、2、3、4、5、6、7 d提取全脑组织，制成匀浆浸渍液，测定脑组织浸渍液EC及其相关生化指标（钾离子、钠离子、氯离子、钙离子、无机磷、镁离子、尿酸、尿素氮及肌酐），并分析浸渍液EC、相关生化指标以及PMI三者之间的关系。 结果 大鼠死后7 d内脑组织EC随PMI的延长而逐渐增大，其浸渍液中尿酸、尿素氮、无机磷的含量随PMI的延长而逐渐升高。EC、尿酸、尿素氮、无机磷与PMI的相关性均较好（R2为0.95~0.99），且EC与尿酸、尿素氮及无机磷的含量变化呈线性相关（R2为0.97~0.99）。其他6种生化指标在大鼠死后7 d内随PMI的变化不显著（P>0.05）。 结论 大鼠脑组织EC、尿素氮、尿酸及无机磷与PMI的相关性较好，联合多指标可以提高PMI推断的准确性。

关键词: 法医病理学, 死亡时间, 电导率, 脑, 生化指标, 大鼠

Abstract: Objective To explore the relationship between the electrical conductivity （EC） and biochemical indicators of rat cerebrum tissues and postmortem intervals （PMIs） and discuss the mechanism of applying EC to infer PMI. Methods Forty healthy Sprague-Dawley rats were sacrificed and stored in an environment of about 25 ℃. The whole cerebrum tissues of rats were removed respectively at different PMIs of 0, 1, 2, 3, 4, 5, 6, and 7 d, and then made into homogenized impregnation solution. The EC and related biochemical indicators （potassium, sodium, chloride, calcium, inorganic phosphorus, magnesium, uric acid, urea nitrogen and creatinine） in cerebrum tissue impregnation solution were determined, and the relationships among EC in impregnation solution, related biochemical indicators and PMI were analyzed. Results The EC in cerebrum tissues increased gradually with the extension of PMI, and the content of uric acid, urea nitrogen and inorganic phosphorus in its impregnation solution also increased gradually with the extension of PMI. The correlation of EC, uric acid, urea nitrogen, and inorganic phosphorus with PMI was relatively good （R2 was 0.95-0.99）, and there was a linear correlation between the content change of uric acid, urea nitrogen, inorganic phosphorus and EC （R2 was 0.97-0.99）. The changes of the other 6 kinds of biochemical indicators with the extension of PMI within 7 d after the rats’ death were non-significant （P>0.05）. Conclusion The correlation between EC in cerebrum tissues, uric acid, urea nitrogen, inorganic phosphorus and PMI were relatively good, and combining various indicators can also improve the accuracy of PMI estimation.

Key words: forensic pathology, postmortem interval, electrical conductivity, brain, biochemical indicator, rats