法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (6): 645-650.DOI: 10.12116/j.issn.1004-5619.2019.06.001

• 论著 •    下一篇

非典型电流损伤死亡大鼠血清代谢组学变化

李林峰1， 董贺文2， 柳丹凤1， 田甜1， 代号1， 刘敏1   

  1. 1. 四川大学华西基础医学与法医学院，四川 成都 610041； 2. 司法鉴定科学研究院 上海市法医学重点实验室 司法部司法鉴定重点实验室 上海市司法鉴定专业技术服务平台，上海 200063
  • 发布日期:2019-12-25 出版日期:2019-12-28
  • 通讯作者: 刘敏，男，教授，主要从事法医病理学教学、科研和鉴定；E-mail：min8liu@hotmail.com
  • 作者简介:李林峰（1992—），男，博士研究生，主要从事法医病理学研究；E-mail：lmelilinfeng@outlook.com

Metabolomics Changes of Serum in Rats Dying from Untypical Electric Injury

LI Lin-feng1, DONG He-wen2, LIU Dan-feng1, TIAN Tian1, DAI Hao1, LIU Min1   

  1. 1. West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu 610041, China; 2. Shanghai Key Lab of Forensic Medicine, Key Lab of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China
  • Online:2019-12-25 Published:2019-12-28

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摘要： 目的 利用核磁共振氢谱（1H nuclear magnetic resonance spectroscopy，1H NMR）代谢组学方法寻找非典型电流损伤死亡大鼠血清中的差异性代谢产物，为生前非典型电击死的判定以及其与死后即刻电击的鉴别提供依据。 方法 分别制作大鼠非典型电击死模型、死后即刻电击模型、机械性窒息死亡模型、机械性损伤死亡模型和高温损伤死亡模型，对照组大鼠不做任何处理常规处死。取各组大鼠血清进行1H NMR代谢组学技术检测，筛选差异性代谢产物。 结果 非典型电击死组与机械性窒息死亡组、机械性损伤死亡组、高温损伤死亡组、对照组间比较，共筛选出4个具有鉴别价值的化学位移点及其对应的多种代谢物，包含醇类、酚类、糖类、氨基酸类等多种小分子物质；非典型电击死组与死后即刻电击组、对照组间比较，共筛选出8个具有鉴别价值的化学位移点及其对应的多种代谢物，包含糖类、氨基酸类、酯类、核酸等多种小分子物质。 结论 1H NMR代谢组学技术能够发现非典型电流损伤死亡的差异性代谢产物，有望为非典型电流损伤死亡的诊断以及生前电击与死后即刻电击的鉴别提供依据。

关键词: 法医病理学, 代谢组学, 电击伤, 核磁共振波谱法, 血清, 大鼠

Abstract: Objective To study the differential metabolites of serum in rats dying from untypical electric injury by 1H nuclear magnetic resonance （1H NMR）-based metabolomics methods, in order to provide clues for identification of death from antemortem untypical electric injury and instant postmortem electric injury. Methods Models of rats dying from untypical electric injury, instant postmortem electric injury, mechanical asphyxia, mechanical injury, and high temperature injury were established. The rats in control group were executed without any treatment. The serums of rats from every group were detected by 1H NMR-based metabolomics technology to screen differential metabolites. Results The rats dying from untypical electric injury group was compared with those from mechanical asphyxia group, mechanical injury group, high temperature injury group, and control group, respectively. Four chemical shift points with diagnostic value, and their corresponding metabolites were screened. These chemical shift points contained many small molecules, such as alcohols, phenols, sugars, amino acids, etc. The death from untypical electric injury group was compared with those from instant postmortem electric injury group and control group, and then eight chemical shift points with diagnostic value and their corresponding metabolites were screened. These chemical shift points contained small molecules, such as sugars, amino acids, esters, nucleic acids, etc. Conclusion The 1H NMR-based metabolomics technology can identify differential metabolites of serum in rats dying from untypical electric injury, therefore it may provide a basis for the diagnosis of death from untypical electric injury and the identification of antemortem electric injury and instant postmortem electric injury.

Key words: forensic pathology, metabonomics, electric injuries, nuclear magnetic resonance spectroscopy, serum, rats