法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (5): 365-368.DOI: 10.3969/j.issn.1004-5619.2011.05.013

• 技术与应用 • 上一篇    下一篇

Investigator Argus X-12试剂盒在华东汉族人群中的法医学应用评估

张素华，朱如心，李  莉，李成涛   

  1. （司法部司法鉴定科学技术研究所 上海市法医学重点实验室，上海 200063）
  • 发布日期:2011-10-25 出版日期:2011-10-28
  • 通讯作者: 通信作者：李成涛，男，博士，副研究员，主要从事法医遗传学研究；E-mail：lichengtaohla@163.com
  • 作者简介:张素华（1985—），女，江苏常州人，硕士，研究实习员，主要从事法医遗传学研究；E-mail：zsh-daisy@163.com
  • 基金资助:

    国家自然科学基金资助项目（30901701，81172908）

Forensic Application of Investigator Argus X-12 Kit in Han Population from East China

ZHANG SU-HUA, ZHU RU-XIN, LI LI, LI CHENG-TAO   

  1. （Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, P.R.China, Shanghai 200063, China）
  • Online:2011-10-25 Published:2011-10-28

被引次数

18

摘要： 目的 调查Investigator Argus X-12试剂盒中所包含的12个X-STR基因座在华东汉族人群中的遗传学数据，考察其法医学应用价值。 方法 应用Investigator Argus X-12试剂盒对华东地区309名汉族无关个体进行X-STR基因座分型检测，统计分析12个X-STR基因座的频率数据、群体遗传学参数及连锁不平衡状况，并与其他地区汉族人群已有数据进行比较。 结果 12个X-STR基因座在华东汉族人群中均符合Hardy-Weinberg平衡，除基因座DXS10103-DXS10101紧密连锁外，其余基因座间相互独立，杂合度均大于0.5，多态信息含量均大于0.4。除DXS10146频率分布与广东汉族人群差异有统计学意义外，其余基因座上的频率分布差异均无统计学意义。 结论 Investigator Argus X-12试剂盒中所包含的X-STR基因座在华东汉族人群中具有高度多态性，适用于法医学中的个人识别和亲权鉴定案例。

关键词: 法医遗传学, Investigator Argus X-12试剂盒, 华东地区, 汉族

Abstract: Objective To investigate the genetic data of the 12 X-STR included in Investigator Argus X-12 kit and to evaluate the forensic application in Han population from East China. Methods By detecting 309 unrelated individuals with Investigator Argus X-12 kit, allele frequencies, population genetics parameters and the information of linkage disequilibrium of the 12 X-STR were analyzed by statistics and were compared with available data of other Han populations from different regions. Results No deviations from Hardy-Weinberg equilibrium were detected.  Except loci of DXS10103 and DXS10101 linked closely, all other loci were independent while HET exceed 0.5 and PIC all above 0.4. Distributions of allele frequencies of all loci were not significant statistically except for locus of DXS10146 in Han population from Guangdong. Conclusion Loci of Investigator Argus X-12 kit were highly polymorphic in Han population from East China, which is suitable for forensic application in paternity testing and individual identification.

Key words: forensic genetics, Investigator Argus X-12 kit, east China, Han nationality

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