法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (2): 112-114，119.DOI: 10.3969/j.issn.1004-5619.2012.02.009

• 论著 • 上一篇    下一篇

人类岩藻糖基转移酶5特异性分布及其在精细胞的表达与定位

李冯锐1，2，周懿舒1，朱兰卉1，崔洪刚1，王保捷1，丁  梅1，庞  灏1   

  1. （1. 中国医科大学法医学院法医血清教研室，辽宁 沈阳 110001； 2. 包头医学院法医学系，内蒙古 包头 014060）
  • 发布日期:2012-04-25 出版日期:2012-04-28
  • 通讯作者: 通信作者：庞灏，男，博士，教授，博士研究生导师，主要从事法医物证学研究；E-mail：panghao8@gmail.com
  • 作者简介:李冯锐（1981—），女，内蒙古包头人，博士研究生，主要从事法医物证学研究；E-mail：rui6826@yahoo.com.cn
  • 基金资助:

    高等学校博士学科点专项科研基金项目（20102104 110021）

Distribution Specificity of Human Fucosyltransferase 5 and Its Expression and Localization in Spermatids

LI FENG-RUI1,2, ZHOU YI-SHU1, ZHU LAN-HUI1, CUI HONG-GANG1, WANG BAO-JIE1, DING MEI1, PANG HAO1   

  1. （1. Department of Forensic Serology, School of Forensic Medicine, China Medical University, Shenyang 110001, China; 2. Department of Forensic Medicine, Baotou Medical College, Baotou 014060, China）
  • Online:2012-04-25 Published:2012-04-28

被引次数

1

摘要： 目的 研究人类岩藻糖基转移酶5（fucosyltransferase 5，FUT5）的特异性分布及在精细胞的表达与定位。 方法 收集健康志愿者的精液（分离精细胞并提取精细胞膜蛋白）、阴道拭子、唾液及静脉血，应用免疫印迹方法检测FUT5在人类精细胞膜、精浆、阴道液、唾液及血清中的表达量，采用免疫荧光技术检测FUT5在精细胞中的表达与定位。 结果 免疫印迹方法结果显示FUT5在精细胞膜及血清中有较高表达，但在精浆、阴道液及唾液中未被检测到。免疫荧光实验结果显示FUT5主要存在于精细胞头部。表明人类精细胞膜存在一定表达量的特异性FUT5，可采用抗原-抗体反应分离精液和阴道液混合斑中的精细胞。 结论 人类FUT5表现出分布特异性，可应用到法医学性犯罪案件中混合斑的鉴定。

关键词: 法医遗传学, 精细胞, 特异性, 岩藻糖基转移酶类

Abstract: Objective To investigate distribution specificity of human fucosyltransferase 5（FUT5） as well as its expression and localization in spermatids. Methods Human semen, vaginal swab, saliva and venous blood from healthy individuals were collected. The spermatids were isolated and the spermatid membrane protein was then extracted. Expression levels of FUT5 from human spermatid membrane, seminal plasma, vaginal fluid, saliva and serum were detected by immunoblotting technique. The expression and localization of FUT5 in spermatids were analyzed by immunofluorescent method. Results Immunoblotting technique showed that FUT5 was expressed on spermatid membranes and in serum, but not in seminal plasma, vaginal fluid and saliva. The expressed FUT5 on spermatids was mostly localized on head of spermatids by fluorescent microscopy, suggesting that there was certain amount of FUT5 on human spermatid membrane, and the spermatids might be isolated from mixed stains with vaginal fluid by antigen-antibody reaction. Conclusion Human FUT5 shows a characteristic distribution specificity, and this feature may be used for identification of mixed stain involved in criminal sexual offence in future forensic practice.

Key words: forensic genetics, spermatids, specificity, fucosyltransferases

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