法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (6): 451-455.DOI: 10.3969/j.issn.1004-5619.2012.06.014

• 技术与应用 • 上一篇    下一篇

新疆喀什地区维吾尔族18个STR基因座的遗传多态性

张晓红1，2，李  平3，翁玮霞1，刘长晖4，刘  宏4，唐建新1，2，刘  超4   

  1. （1. 南方医科大学法医学系，广东 广州 510515； 2. 广州市公安局番禺分局，广东 广州 511400； 3. 新疆喀什地区公安局，新疆 喀什 844000； 4. 广州市刑事科学技术研究所，广东 广州 510030）
  • 发布日期:2012-12-25 出版日期:2012-12-28
  • 通讯作者: 刘超，男，博士，教授，主任法医师，主要从事DNA多态性法医学应用研究；E-mail：liuchaogaj@21cn.com
  • 作者简介:张晓红（1973—），女，广东潮阳人，硕士研究生，副主任法医师，主要从事DNA多态性法医学应用研究
  • 基金资助:

    广东省重点科技项目（2010A060801001）；新疆维吾尔自治区科技支疆计划项目（201091143）

Genetic Polymorphisms of 18 STR Loci in Uygur Population of Kashi Prefecture of Xinjiang

ZHANG XIAO-HONG1,2, LI PING3, WENG WEI-XIA1, LIU CHANG-HUI4, LIU HONG4, TANG JIAN-XIN1,2, LIU CHAO4   

  1. （1. Department of Forensic Medicine, Southern Medical University, Guangzhou 510515, China; 2. Panyu Branch of Guangzhou Public Security Bureau, Guangzhou 511400, China; 3. Kashi Public Security Bureau of Xinjiang, Kashi 844000, China; 4. Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China）
  • Online:2012-12-25 Published:2012-12-28

摘要： 目的 调查新疆喀什地区维吾尔族无关个体18个STR基因座（D18S51、D21S11、D3S1358、FGA、D8S1179、vWA、CSF1PO、D16S539、D7S820、D13S317、D5S818、D2S1338、D19S433、D12S391、TPOX、TH01、Penta E和D6S1043）的遗传多态性并研究其法医学应用价值。 方法 采用DNA TyperTM 15 Plus试剂盒对1 381名维吾尔族无关个体血样进行复合扩增，3130XL遗传分析仪对扩增产物检测，GeneMapper ID v3.2软件进行基因分型。计算群体遗传学参数，并与其他人群进行比较，计算Reynold’s遗传距离，绘制系统发生树。 结果 在1 381名维吾尔族无关个体中，共发现231种等位基因，单个等位基因频率分布在0.000 4~0.530 4，H在0.644~0.923，PIC在0.587~0.918，DP在0.817~0.988，CPE大于0.999 999 9。与广州汉族人群遗传距离最大（0.088 3），与希腊人群遗传距离最小（0.050 3）。 结论 这18个STR基因座在新疆喀什地区具有高度多态性，可满足该群体个体识别和亲权鉴定，其遗传多态性更接近欧洲。

关键词: 法医遗传学, 短串联重复序列, 多态现象, 遗传, 新疆[维吾尔自治区], 维吾尔族

Abstract: Objective To investigate the genetic polymorphisms of 18 STR loci （D18S51, D21S11, D3S1358, FGA, D8S1179, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818, D2S1338, D19S433, D12S391, TPOX, TH01, Penta E and D6S1043） in unrelated Uygur individuals in Kashi prefecture of Xinjiang and to explore the application value in forensic practice. Methods Blood samples from 1 381 unrelated Uygur individuals were amplified by using DNA TyperTM 15 Plus kit. The amplified products were detected by using 3130XL Genetic Analyzer and the genotyping was done by using GeneMapper ID v3.2. Population genetics parameters were calculated and compared with that of the other population. The genetic distance of Reynold’s was calculated and phylogenetic tree was constructed at last. Results Of the 1 381 unrelated Uygur individuals, 231 alleles were detected, with an allele frequency of 0.000 4-0.530 4. The H values were 0.644-0.923, PIC values were 0.587-0.918, and DP values were 0.817-0.988, respectively, with a CPE>0.999 999 9. The genetic distance was the longest （0.088 3） to Guangzhou Han population and the closest （0.050 3） to Greek. Conclusion The 18 STR loci in the Uygur population of Kashi prefecture of Xinjiang have high genetic polymorphisms which are close to Europeans, and can be satisfied as genetic markers of population individual identification and paternity testing.

Key words: forensic genetics, STR, polymorphism, genetic, Xinjiang, Uygur nationality

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